Eric Scott Nordman

US Patent:

6372106, Apr 16, 2002

Filed:

Jul 26, 1999

Appl. No.:

09/361485

Inventors:

Ben F. Johnson - Palo Alto CA
Eric S. Nordman - Palo Alto CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 2726

US Classification:

204455, 204451, 204601, 204605

Abstract:

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

US Patent:

6387236, May 14, 2002

Filed:

Mar 13, 2001

Appl. No.:

09/805624

Inventors:

Eric S. Nordman - Palo Alto CA
John Shigeura - Fremont CA
Albert L. Carrillo - Redwood City CA
David M. Demorest - Soquel CA
Philip J. Wunderle - El Sobrante CA

Assignee:

PE Corporation (NY) - Foster City CA

International Classification:

G01N 2726

US Classification:

204601, 204451, 204452, 204453, 204603, 204604, 422 99, 422100, 422104

Abstract:

A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

US Patent:

6596140, Jul 22, 2003

Filed:

May 1, 2001

Appl. No.:

09/846855

Inventors:

Eric S. Nordman - Palo Alto CA
Richard T. Reel - Hayward CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 27447

US Classification:

204452, 204461, 204603, 204612

Abstract:

Embodiments of a device and method are described which provide for control over the distortion of a sample zone upon exiting an electrophoresis separation channel. According to the teachings herein (i) the downstream regions of the channels, near the outlet ends, and/or (ii) the detection chamber, is/are configured so that distortion of one or more sample zones passing from the channels into and across the detection chamber can be controlled (e. g. , reduced) in a fashion affording enhanced detectability. In certain embodiments, the lumens along the end regions of the separation channels progressively expand.

US Patent:

6856390, Feb 15, 2005

Filed:

Jul 25, 2002

Appl. No.:

10/205028

Inventors:

Eric S. Nordman - Palo Alto CA, US
Richard T. Reel - Hayward CA, US
John Shigeura - Portola Valley CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N021/00

US Classification:

356344

Abstract:

An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.

US Patent:

7014744, Mar 21, 2006

Filed:

Aug 24, 2001

Appl. No.:

09/938894

Inventors:

Richard T. Reel - Hayward CA, US
Eric S. Nordman - Palo Alto CA, US
Zbigniew T. Bryning - Campbell CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 27/26
C12N 13/00

US Classification:

204600, 204643, 4351739

Abstract:

Embodiments of methods and devices are disclosed for the manipulation (e. g. , concentration, purification, capture, trapping, location, transfer, etc. ) of analytes, e. g. , biomolecules, with respect to analyte-containing solutions using one or more electric fields.

US Patent:

7169276, Jan 30, 2007

Filed:

Feb 13, 2002

Appl. No.:

10/075404

Inventors:

Ben F. Johnson - Palo Alto CA, US
Eric S. Nordman - Palo Alto CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 27/447

US Classification:

204451, 204455

Abstract:

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

US Patent:

7169279, Jan 30, 2007

Filed:

Nov 4, 2002

Appl. No.:

10/288405

Inventors:

Howard Gregg King - Berkeley CA, US
John Shigeura - Portola Valley CA, US
Eric S. Nordman - Palo Alto CA, US
Sean Matthew Desmond - San Carlos CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 27/447

US Classification:

204601, 204451, 204604, 204453

Abstract:

A sample handling system in a multi-channel capillary electrophoresis apparatus is disclosed. The sample handling system includes a work surface for supporting a plurality of samples located at a plurality of work surface coordinates and a sample loading assembly comprising a plurality of loading wells. At least one of the loading wells includes a capillary fixedly positioned therein. The system further includes a programmable sample transfer device for automatically transferring a sample from a work surface coordinate to a loading well. The invention further includes methods for using the sample handling system.

US Patent:

7177023, Feb 13, 2007

Filed:

Mar 19, 2004

Appl. No.:

10/805096

Inventors:

Richard T. Reel - Hayward CA, US
Eric S. Nordman - Palo Alto CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01J 3/30

US Classification:

356317

Abstract:

System and method for fluorescent light detection from biological samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.