Eric W Triplett

US Patent:

7141395, Nov 28, 2006

Filed:

Aug 10, 2001

Appl. No.:

09/927616

Inventors:

Eric W. Triplett - Middleton WI, US
Thomas C. Herlache - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12P 21/04
C12N 1/20
A01N 63/00
A61K 48/00

US Classification:

435 713, 4352522, 4352523, 424 932, 424 934

Abstract:

The present invention provides a method for controlling crown gall disease in plants using an effective quantity of α-proteobacteria that produces trifolitoxin (TFX). The present invention also provides a biocontrol agent for use in the above method, and a plant coated with the biological control agent. The biocontrol agent is characterized as a biologically pure culture of an α-proteobacteria strain that produces TFX, or an α-proteobacteria strain genetically engineered to produce TFX. The α-proteobacteria strain employed may include any one of the many strains of capable of producing crown galls, including and, in particular, F2/5. The α-proteobacteria strain employed may be genetically engineered to produce TFX by introducing a genetic construct into the so as to cause the to carry and express the tfx operon from. The bacteria may also be genetically engineered to produce TFX by introducing a pT2TFXK plasmid into the. The biocontrol agent may also be the strain F2/5 (pT2TFXK), ATCC Patent Deposit Designation PTA-2356.

US Patent:

20020142917, Oct 3, 2002

Filed:

Dec 3, 2001

Appl. No.:

10/005412

Inventors:

Eric Triplett - Middleton WI, US
Shawn Kaeppler - Oregan WI, US
Marisa Chelius - Greeley CO, US

International Classification:

A01N063/00
A01N025/26
C12N001/12
C12N001/20

US Classification:

504/117000, 504/100000, 435/252100

Abstract:

A biological inoculant for enhancing the growth of plants is disclosed. The inoculant includes the bacterial strains 2A, P101, P102, 342, zmvsy, Z152, PA15, with or without a carrier. The inoculant also includes strains of the bacterium and which are able to enhance the growth of cereal grasses. Also disclosed are the novel bacterial strains 2A, P101 and P102, and 342 and zmvsy.

US Patent:

20090137390, May 28, 2009

Filed:

Jun 30, 2005

Appl. No.:

11/630844

Inventors:

Eric Wendell Triplett - Gainesville FL, US

International Classification:

A01N 63/00
A01H 5/00

US Classification:

504117, 800301

Abstract:

The subject invention concerns materials and methods for providing or enhancing nitrogen fixation in plants. The invention provides for the use of nitrogen fixing bacteria that are isolated from nitrogen efficient plants. Plants for which enhanced nitrogen fixation is desired are inoculated with an effective amount of nitrogen firing bacteria of the invention. In an exemplified embodiment, the bacteria is Kp342. The subject invention also concerns means to increase the number of free-living nitrogen-fixing bacteria in plants. Mutants of beneficial endophytic bacteria that are resistant to plant defense responses can be used to colonize a plant in numbers higher than a wild type or a non-mutated bacteria can colonize a plant. The higher number of bacteria colonizing the plant provide for more nitrogen fixation for the plant. The subject invention concerns methods for producing non-leguminous plants that are capable e of utilizing atmospheric nitrogen by colonization with a nitrogen fixing endophyic bacteria that is resistant to plant defense responses. The subject invention also concerns the plants produced by the subject method. The subject invention also concerns methods for producing the mutant endophytic bacteria. The subject invention also concerns the mutant endophytic bacteria produced using the subject methods.

US Patent:

20120183513, Jul 19, 2012

Filed:

Feb 18, 2010

Appl. No.:

13/144028

Inventors:

Josef Neu - Gainesville FL, US
Graciela Liliana Lorca - Gainesville FL, US
Eric W. Triplett - Gainesville FL, US
Mark A. Atkinson - Gainesville FL, US
Desmond A. Schatz - Gainesville FL, US

International Classification:

A61K 35/74
A61P 1/14
C12N 1/20
A61P 3/10
A61P 37/04

US Classification:

424 9344, 424 9345, 424 934, 4352529

Abstract:

Isolated strains are useful in preventing or delaying the development of Type 1 Diabetes (T1D). A probiotic composition comprising the strains and use of the composition in T1D prevention are provided.

US Patent:

58587624, Jan 12, 1999

Filed:

May 1, 1997

Appl. No.:

8/848785

Inventors:

Eric W. Triplett - Madison WI

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 120

US Classification:

4352523

Abstract:

A plasmid for use in root nodule bacteria is described which is intended both to increase yield of inoculated legume plants inoculated with the bacteria as well as aiding in the competitiveness of the bacteria with native strains. The plasmid include a hydrogen uptake element which aids in energy efficient nitrogen fixation by the plant bacteria symbiotic pair as well as a toxin and resistance element intended to aid in the competitive fitness of the inoculant bacterial strain.

US Patent:

59069295, May 25, 1999

Filed:

May 1, 1997

Appl. No.:

8/847207

Inventors:

Eric W. Triplett - Madison WI

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 1563
C12N 121
C12N 1581
C07H 2104

US Classification:

4351723

Abstract:

Root nodule bacterial strains are designed specifically to aid in the growth of soybeans. A high copy number plasmid is described to make trifolitoxin, an antibiotic. The plasmid is preferably hosted in a Sinorhizobium species which is capable of nodulating roots of soybean plants. The phenotype of trifolitoxin production confers a competitive advantage on the inoculant strains by inhibiting competitive strains. To facilitate soybean growth a separate hydrogen uptake capability is also included in the inoculant, either in the same Sinorhizobium strain or in a companion trifolitoxin-resistant strain of root nodule bacteria introduced in the same inoculant.

US Patent:

51837595, Feb 2, 1993

Filed:

May 22, 1991

Appl. No.:

7/707245

Inventors:

Eric W. Triplett - Madison WI

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12R 141
C12P 2100
C12P 2104
C12N 1500

US Classification:

4352522

Abstract:

The sequences of a Rhizobium bacteria responsible for competitiveness with respect to plant nodulation have been isolated and permanently transferred to superior nodulating Rhizobium genome. This has resulted in a stable construct that can form a plant inoculant that yields effective nodulation, while reducing the risk of suppression by other bacteria in the environment.

US Patent:

59087587, Jun 1, 1999

Filed:

Jun 19, 1997

Appl. No.:

8/879128

Inventors:

Eric W. Triplett - Madison WI

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12Q 124
C12Q 100
C12Q 168
C12N 1511

US Classification:

435 30

Abstract:

A novel category of microorganisms has been identified from its ribosomal DNA (rDNA) sequence. That sequence, P36, is characteristic of this category of microorganisms, here termed metathermophiles. Methods for isolating samples of metathermophiles using the rDNA sequence are described.