Erik A Gustafson

US Patent:

7527929, May 5, 2009

Filed:

Jul 29, 2005

Appl. No.:

11/194072

Inventors:

Kevin J. McKernan - Marblehead MA, US
Erik Gustafson - Norwood MA, US
Adrianne D. Brand - Wenham MA, US

Assignee:

Agencourt Bioscience Corporation - Beverly MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention is directed to a method of isolating a target species (e. g. , target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e. g. , buffer) that selectively removes (e. g.

US Patent:

20090191566, Jul 30, 2009

Filed:

Mar 3, 2009

Appl. No.:

12/396586

Inventors:

Kevin J. McKernan - Marblehead MA, US
Erik Gustafson - Norwood MA, US
Adrianne D. Brand - Wenham MA, US

Assignee:

AGENCOURT BIOSCIENCE CORPORATION - Beverly MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g., elutes) either the nucleic acid from the first functional group or the target nucleic acid species from the second functional group of the solid phase carriers, thereby isolating a target nucleic acid species from a mixture comprising a plurality of nucleic acid species.

US Patent:

20130232997, Sep 12, 2013

Filed:

Mar 6, 2012

Appl. No.:

13/413104

Inventors:

Erik Gustafson - Waleska GA, US

International Classification:

F17C 7/04

US Classification:

62 481

Abstract:

Described herein are systems and methods for cryogenic fluid delivery. The systems may include a pressure vessel containing a cryogenic fluid formed of liquid and vapor that is connected to a use device via a withdrawal line. The withdrawal line connects to the cryogenic fluid in the pressure vessel via two routes, a liquid tube and a vapor line. The vapor line may include a back-pressure regulator that opens the vapor line depending on pressure in the system. The withdrawal line may include a pressure relief valve that exerts pressure on the liquid tube. A bypass line may connect the withdrawal line to the liquid tube. The bypass line has a check valve that permits free flow of cryogen from the withdrawal line to the liquid tube via the bypass line while prohibiting cryogen flow from the pressure vessel through the bypass line. The methods employ the systems described herein.

US Patent:

20190277450, Sep 12, 2019

Filed:

Mar 6, 2019

Appl. No.:

16/293985

Inventors:

- Ball Ground GA, US
Erik Gustafson - Waleska GA, US

International Classification:

F17C 7/02
F17C 5/02

Abstract:

A system for transferring cryogenic fluid from a dispensing tank to a receiving tank is disclosed. The dispensing tank stores a supply of cryogenic liquid with a dispensing tank headspace above the liquid. A compressor has an inlet connected to the headspace of a receiving tank and an outlet connected to the headspace of the dispensing tank. A liquid transfer line is in fluid communication with the liquid side of the dispensing tank and the receiving tank. Cryogenic liquid is transferred from the dispensing tank to the receiving tank when the compressor is activated so as to transfer vapor from the headspace of the receiving tank to the headspace of the dispensing tank to create a pressure differential between the dispensing and receiving tanks.

US Patent:

20170140096, May 18, 2017

Filed:

Nov 11, 2016

Appl. No.:

15/349868

Inventors:

- Brea CA, US
Zihua Wang - Newton MA, US
Antonio Arevalo Reyes - Middleton MA, US
Erik Alan Gustafson - Alpharetta GA, US
John Steven Riley - Miami FL, US

International Classification:

G06F 19/24
C12Q 1/68
G06F 19/18
G06F 19/12

Abstract:

A method for determining the presence of a biological entity. The method may include entering into a digital computer, at least a plurality of first input values associated with a first genetic element, a plurality of second input values associated with a second genetic element, and a plurality of third input values associated with a third genetic element associated with a plurality of samples. The method also includes determining a threshold value associated with the third genetic element, separating the samples using the threshold value into a first set of samples and a second set of samples, clustering the first set of samples in a feature space defined by the first genetic element and the second genetic element, defining a first boundary space using the first set of samples, and defining a second boundary space using the second set of samples.

US Patent:

20140336143, Nov 13, 2014

Filed:

Dec 4, 2013

Appl. No.:

14/096166

Inventors:

- Foster City CA, US
- Boston MA, US
- Atlanta GA, US
Erik Gustafson - Norwood MA, US

Assignee:

GILEAD PHARMASSET LLC - Foster City CA
BETH ISRAEL DEACONESS MEDICAL CENTER - Boston MA
EMORY UNIVERSITY - Atlanta GA

International Classification:

C07H 19/06
C07D 409/04
C07D 405/04
C07D 239/54

US Classification:

514 50, 514274

Abstract:

A method and composition for the treatment, prevention and/or prophylaxis of a host, and in particular, a human, infected with Epstein-Barr virus (EBV), is provided that includes administering an effective amount of a 5-substituted uracil nucleoside or its pharmaceutically acceptable salt or prodrug, optionally in a pharmaceutically acceptable diluent or excipient.

US Patent:

20140223924, Aug 14, 2014

Filed:

Feb 11, 2014

Appl. No.:

14/177781

Inventors:

- GARFIELD HEIGHTS OH, US
Erik Gustafson - Waleska GA, US
Jeff Patelczyk - Cumming GA, US
David Davis - Canton GA, US
Michael Sable - Canton GA, US
Luis Serentill - Lawrenceville GA, US
Peter Murray - Cumming GA, US

Assignee:

CHART INC. - GARFIELD HEIGHTS OH

International Classification:

F17C 13/00

US Classification:

62 451

Abstract:

An integrated cryogenic fluid delivery system includes a cryogenic liquid tank having an interior, a wall and a geometry. The interior of the cryogenic liquid tank contains a supply of cryogenic liquid. A fuel pickup line is positioned within the interior of the tank and is in fluid communication with a vaporizer so that the vaporizer receives and vaporizes cryogenic liquid from the tank. The vaporizer is positioned outside of the tank and is secured to the wall. The vaporizer also has a shape that conforms with the geometry of the tank.