Erin K Heiniger

US Patent:

20210349087, Nov 11, 2021

Filed:

Oct 1, 2019

Appl. No.:

17/278166

Inventors:

- Seattle WA, US
Joshua BISHOP - Seattle WA, US
Dylan GUELIG - Seattle WA, US
Arielle HOWELL - Seattle WA, US
Sujatha KUMAR - Seattle WA, US
Paul YAGER - Seattle WA, US
Koji ABE - Seattle WA, US
Erin HEINIGER - Seattle WA, US
Samantha BYRNES - Seattle WA, US
Caitlin ANDERSON - Seattle WA, US
Peter C. KAUFFMAN - Seattle WA, US
Maxwell WHEELER - Seattle WA, US

Assignee:

University of Washington - Seattle WA

International Classification:

G01N 33/558
B01L 3/00
G01N 33/543

Abstract:

The present technology generally relates to stopped-flow microfluidic devices. Select embodiments of the present technology include microfluidic devices having a first porous element configured to receive a first fluid and a second porous element configured to receive a second fluid. The second porous element can have one or more legs overlapping with the first porous element. The device can be configured such that (a) delivery of the first fluid to the first porous element causes the first fluid to flow along the length of the first porous element without substantially wetting the one or more legs, and (b) delivery of the second fluid to the second porous element causes the second fluid to flow into the overlapping regions of the first porous element, thereby substantially stopping flow of the first fluid along at least a portion of the first porous element.

US Patent:

20180119202, May 3, 2018

Filed:

Nov 1, 2017

Appl. No.:

15/801223

Inventors:

- Seattle WA, US
Joshua Bishop - Seattle WA, US
Erin Heiniger - Seattle WA, US

International Classification:

C12Q 1/68
G01N 33/543

Abstract:

The present technology is related to methods and compositions for detecting, and optionally quantifying, one or more analytes of a sample using nucleic acids. In some embodiments, the methods include generating a complex of a plurality of peptides, an analyte, a first nucleic acid, and a second nucleic acid, each nucleic acid conjugated to a binder peptide. In addition, an immobilizer peptide can be immobilized to a substrate. If the binder peptides are bound to the analyte, the method further includes hybridizing a segment of the first nucleic acid to a segment of the second nucleic acid and amplifying the hybridized nucleic acids to generate a plurality of amplicons. Moreover, the generated amplicons indicate that one or more analytes has been detected. A number of generated amplicons can be analyzed to quantify one or more of the bound analytes.

US Patent:

20170131211, May 11, 2017

Filed:

Nov 10, 2016

Appl. No.:

15/348926

Inventors:

- Seattle WA, US
Joshua Bishop - Seattle WA, US
Joshua Buser - Seattle WA, US
Louise Lyth Hansen - Seattle WA, US
Erin K. Heiniger - Seattle WA, US
Enos Kline - Seattle WA, US
Sujatha Kumar - Seattle WA, US

International Classification:

G01N 21/64
C12Q 1/68

Abstract:

The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, the system includes a vessel configured to receive a biological sample and a cap assembly that includes a porous membrane having a receiving region and a detection region. When the cap assembly is detachably coupled to an open end portion of the vessel, the system can be moved between a first orientation and a second orientation. When the system is in the first orientation, the biological sample is not in fluid communication with the receiving region. When the vessel contains is in the second orientation, the biological sample is in fluid communication with the receiving region and wicks through the porous membrane to the detection region.

US Patent:

20160310942, Oct 27, 2016

Filed:

Apr 21, 2016

Appl. No.:

15/135461

Inventors:

- Seattle WA, US
Joshua Buser - Seattle WA, US
Samantha Byrnes - Seattle WA, US
Erin K. Heiniger - Seattle WA, US
Peter C. Kauffman - Bainbridge Island WA, US
Paula Ladd - Seattle WA, US

International Classification:

B01L 3/00
C12Q 1/68

Abstract:

The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a first porous element having a first pore size and configured to receive a fluid at its proximal portion, and a second porous element having a second pore size greater than the first pore size and configured to receive a fluid at its proximal portion. The first porous element can be positioned across the second porous element such that an overlapping region exists between the porous elements where the porous elements are in fluid communication. Before delivery of the fluid to the second porous element, the fluid pressure at the overlapping region is greater than the capillary pressure of the second porous element such that a fluid delivered to the first porous element wicks through its overlapping portion without wetting the second porous element.

US Patent:

20150203806, Jul 23, 2015

Filed:

Jan 21, 2015

Appl. No.:

14/601966

Inventors:

- Seattle WA, US
Peter C. Kauffman - Bainbridge Island WA, US
Joshua Buser - Seattle WA, US
Samantha Byrnes - Seattle WA, US
Alec K. Wollen - Seattle WA, US
Erin Heiniger - Seattle WA, US

International Classification:

C12M 1/00
C12M 1/42
C12N 13/00

Abstract:

The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, system includes a vessel configured to receive the biological sample, a permanent magnet configured to be positioned within the vessel, an electromagnet configured to be positioned proximate the vessel, and a current source operably coupled to the electromagnet and configured to transmit an alternating current. In some embodiments, when the biological sample is placed within the vessel and the alternating current is transmitted to the electromagnet, the electromagnet produces an alternating magnetic field that causes the permanent magnet to rotate within the vessel, thereby lysing at least one of the cells of the biological sample.