Eugene J Young

US Patent:

6965105, Nov 15, 2005

Filed:

Mar 13, 2003

Appl. No.:

10/387755

Inventors:

Mark F. Oldham - Los Gatos CA, US
Eugene F. Young - Marietta GA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

H01J003/14
H01J040/14
H01J005/16
G01N021/64

US Classification:

250236, 356317, 356417, 2504581, 2504591, 2504561, 2504562

Abstract:

A system for detecting fluorescence emitted from a plurality of samples in a sample tray is provided. The system generally includes a plurality of lenses positioned in a linear arrangement, a linear actuator configured to translate the plurality of lenses, an excitation light source for generating an excitation light, an excitation light direction mechanism for directing the excitation light to a single lens of the plurality of lenses at a time so that a single sample holder aligned with the lens is illuminated at a time, and an optical detection system for analyzing light from the sample holders. In certain embodiments, the optical detection system includes a light dispersing element configured to spectrally disperse the light from the sample holder being illuminated, and a lens element configured to receive light from the light dispersing element and direct the light onto a light detection device. A method of scanning a sample tray having a plurality of samples positioned in sample holders to detect fluorescence is also provided.

US Patent:

7387891, Jun 17, 2008

Filed:

May 19, 2003

Appl. No.:

10/440719

Inventors:

Steven J. Boege - San Mateo CA, US
Mark F. Oldham - Los Gatos CA, US
Eugene F. Young - Marietta GA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34
C12M 3/00
G01N 21/64
G01N 21/66
F21V 9/16

US Classification:

4352887, 4352884, 435808, 422 8208, 378 42, 378 45, 2504831, 2504591, 2504612

Abstract:

An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with an excitation beam generated by a light source. A collimating lens can be disposed along a beam path between the light source and the reaction regions to form bundles of collimated excitation beams, wherein each bundle corresponds to a respective reaction region. Methods of analysis using the optical instrument are also provided.

US Patent:

7410793, Aug 12, 2008

Filed:

May 19, 2003

Appl. No.:

10/440920

Inventors:

Steven J. Boege - San Mateo CA, US
Howard G. King - Berkeley CA, US
Eugene F. Young - Marietta GA, US
Johannes P. Sluis - El Granada CA, US
Mark F. Oldham - Los Gatos CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34
C12M 3/00
C12M 1/00
G01N 21/64
G01N 21/66

US Classification:

4352887, 435808, 435809, 4353031, 422 8208, 2504612, 2504611, 356 73

Abstract:

An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

US Patent:

7498164, Mar 3, 2009

Filed:

Jun 6, 2003

Appl. No.:

10/456196

Inventors:

Mark F. Oldham - Los Gatos CA, US
Eugene F. Young - Marietta GA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12M 1/34
C12M 3/00
G01N 21/64
G01J 1/58
F21V 9/16

US Classification:

4352887, 435808, 4353031, 4352884, 435809, 378 42, 378 45, 422 8208, 2504831, 2504591, 2504611, 2504612, 356 73

Abstract:

An instrument is provided that can monitor nucleic acid sequence amplifications reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

US Patent:

7635588, Dec 22, 2009

Filed:

May 19, 2003

Appl. No.:

10/440852

Inventors:

Howard G. King - Berkeley CA, US
Steven J. Boege - San Mateo CA, US
Eugene F. Young - Marietta GA, US
Mark F. Oldham - Los Gatos CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12M 1/34
C12M 3/00
F21V 9/16
G01J 1/58

US Classification:

4352887, 435808, 4353031, 4352884, 435809, 2504591, 2504611, 2504612, 2504621, 2504831, 378 42, 378 45, 356 73

Abstract:

An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.

US Patent:

8492138, Jul 23, 2013

Filed:

Sep 12, 2012

Appl. No.:

13/611462

Inventors:

Steven J. Boege - San Mateo CA, US
Howard G. King - Berkeley CA, US
Eugene F. Young - Marietta GA, US
Johannes P. Sluis - Redwood City CA, US
Mark F. Oldham - Emerald Hills CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12M 1/34
C12M 3/00

US Classification:

4352887, 435808, 435809, 2504591, 2504611, 2504612, 2504621, 2504831

Abstract:

An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

US Patent:

8557569, Oct 15, 2013

Filed:

Jul 3, 2008

Appl. No.:

12/217410

Inventors:

Steven J. Boege - San Mateo CA, US
Howard G. King - Berkeley CA, US
Eugene F. Young - Marietta GA, US
Johannes P. Sluis - El Granada CA, US
Mark F. Oldham - Los Gatos CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12M 3/00
C12M 1/34
F21V 9/16

US Classification:

4352887, 435808, 435809, 2504591, 2504612, 2504621, 2504831, 2504611

Abstract:

An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

US Patent:

8591836, Nov 26, 2013

Filed:

Apr 8, 2008

Appl. No.:

12/099644

Inventors:

Steven J. Boege - San Mateo CA, US
Eugene F. Young - Marietta GA, US
Donald R. Sandell - San Jose CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

B01L 3/00

US Classification:

422552, 422551, 422553

Abstract:

A cover for a biological sample well tray, comprising a cap for sealing a sample well. The cap comprises a well lens for focusing light into the sample well and collecting light from the sample. In another aspect, the cap comprises an elongate portion configured to permit incoming light to pass into the sample well and out of the sample well. Various other aspects comprise a microcard for biological material, and an apparatus for a plurality of sample well strips. A method for testing a biological sample is also provided.