Skills:
Molecular biology lab techniques: RNA extraction, PCR, Taqman and SYBR green based qRT-PCR, cloning, cell transfection, western blot, mutagenesis PCR array, Microarray data analysis, Gene Set Enrichment Analysis, construction of mutant Plasmodium strains. In vivo techniques: Handling of small rodent animals Adoptive cell transfer, construction of bone marrow chimeras Intravital imaging (spinning disk confocal), experience with mouse model of infection with biosafety level 2 (BL2) pathogens such as vaccinia and Plasmodium. Immunology and in vitro techniques: Multi-parameter flow cytometry (BD FACS Calibur, Canto, LSRII) and analysis (Flowjo, DIVA, SPICE/PESTLE), iImmunofluoresence, MACS cell isolation/sorting, ELISA, proliferation assay, in vitro T cell activation, maintenance of cell lines, tissue culture, plaque assays. COMPUTER SKILLS: Partek Genomic Suite Analysis, Word processing, spreadsheets, Powerpoint, GraphPad Prism, Adobe Photoshop, Illustrator, Inkscape, GIMP, Endnote, Zotero and Mendeley