Feng Yang

US Patent:

20110059455, Mar 10, 2011

Filed:

Sep 2, 2010

Appl. No.:

12/874602

Inventors:

Feng Yang - Acton MA, US
Sha-Sha Wang - Wellesley MA, US
Laurence Michael Vaughan - Cockeysville MD, US
Michael Porter - Baltimore MD, US
Elaine Rose - Raleigh NC, US

Assignee:

BECTON, DICKINSON AND COMPANY - Franklin Lakes NJ

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435 6, 435 792

Abstract:

A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

Address:

318 Stevenson Ln APT C4, Towson, MD 21204

VIN:

1HGCM56147A129444

Make:

HONDA

Model:

ACCORD

Year:

2007