Bruce D. Hammock - Davis CA Ferenc Szurdoki - Davis CA Horacio Kido - Davis CA
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
G01N 3320 G01N G01N G01N 3358
US Classification:
435 71
Abstract:
Methods of assaying for the presence or amount of a metal ion in a sample suspected of containing such ions. In one aspect, an enzyme amplified sandwich assay is provided which relies upon the ability of the analyte (metal ion) to form a complex with two complexing agents (chelators). In this assay, the first sandwich chelator is immobilized on a solid support, while the second sandwich chelator is linked to a reporter group (e. g. , an enzyme). This assay combines the specific recognition of the analyte by the first and second sandwich chelators with the great signal amplification offered by the reporter group (e. g. , enzyme). In another aspect, a competitive assay is provided that relies on the competitive inhibition of complex formation between the coating ligand (i. e. , the chelator attached to the solid support) and the organometallic compound attached to the reporter group (e. g. , enzyme) by the metal ions of interest present in the sample.
Massachusetts College of Pharmacy and Health Sciences since Sep 2010
Adjunct Instructor in Chemistry
Immunetics, Inc. 2004 - 2008
Scientist
Minerva Biotechnologies Corporation 2002 - 2004
Senior Chemist
Polestar Technologies, Inc. 2000 - 2001
Senior Scientist
Tufts University 1997 - 2000
Research Associate
Education:
Technical University of Budapest
Skills:
Elisa Protein Chemistry Chemistry Assay Development Immunoassays Fluorescence Fluorescence Microscopy