Frank T Robb

US Patent:

6579703, Jun 17, 2003

Filed:

Apr 16, 2001

Appl. No.:

09/835909

Inventors:

Frank T. Robb - Silver Spring MD
Pongpan Laksanalamai - Baltimore MD

Assignee:

University of Maryland Biotechnology Institute - Baltimore MD

International Classification:

C12D 1934

US Classification:

435 911, 435 6, 435 732, 435 711, 435 912, 530350

Abstract:

Small heat shock proteins, e. g. , (Pfu-sHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e. g. , Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.

US Patent:

7247432, Jul 24, 2007

Filed:

Mar 17, 2003

Appl. No.:

10/390292

Inventors:

Frank T. Robb - Gaithersburg MD, US
Pongpan Laksanalamai - Baltimore MD, US

Assignee:

University of Maryland Biotechnology Institute - Baltimore MD

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Small heat shock proteins, e. g. , (Pfu-sHSP and/or Pfu-tsHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e. g. , Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.

US Patent:

20080318281, Dec 25, 2008

Filed:

Aug 29, 2006

Appl. No.:

12/065152

Inventors:

Frank T. Robb - Gaithersburg MD, US

Assignee:

UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE - Baltimore MD

International Classification:

C12P 19/34
C07K 14/00

US Classification:

435 912, 530350

Abstract:

The present invention relates to maintaining the activity and stability of enzymes and biologically active proteins at increased temperatures by contacting same with a combination of isolated passive and active chaperones from a hyperthermopilic Archaeon, wherein the chaperones may include heat shock proteins, prefoldin and/or chaperonin proteins.

US Patent:

20110045554, Feb 24, 2011

Filed:

Feb 26, 2008

Appl. No.:

12/528620

Inventors:

Douglas S. Clark - Orinda CA, US
Timothy Whitehead - Berkeley CA, US
Frank T. Robb - Gaithersburg MD, US
Pongpan Laksanalamai - Baltimore MD, US
Anchalee Jiemjit - Baltimore MD, US

Assignee:

The Regents of the University of California - Oakland CA
University of Maryland, Baltimore - Baltimore MD

International Classification:

C12P 7/64
C12P 1/00
C12P 7/26
C12P 7/16
C12P 7/06
C12P 5/00
C12P 1/04
C12P 1/02
C12N 15/63
C12N 1/00
C12N 1/21
C12N 1/15
C12N 1/19
C12N 1/13
C12N 15/81
C12N 15/74
C12N 15/80
C12N 15/70

US Classification:

435134, 435 41, 435148, 435160, 435161, 435166, 435170, 435171, 435471, 435243, 4352523, 43525231, 43525232, 43525233, 43525411, 4352542, 43525421, 43525422, 43525423, 4352543, 4352572

Abstract:

The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.

US Patent:

20120064560, Mar 15, 2012

Filed:

Jun 16, 2009

Appl. No.:

12/999185

Inventors:

Harold E. Smith - Bethesda MD, US
Frank T. Robb - Gaithersburg MD, US

International Classification:

C12Q 1/02
C07H 21/04
C12N 1/21

US Classification:

435 29, 43525233, 536 237

Abstract:

The present invention relates to recombinant protein production, and more specifically, to methods for recovery of properly folder bioactive proteins by expressing chaperone genes from extremophilic Archaea, during recombinant protein synthesis in a host cell thereby significantly improving recovery of properly folded bioactive proteins.

US Patent:

56100663, Mar 11, 1997

Filed:

Dec 10, 1993

Appl. No.:

8/166346

Inventors:

Carl W. Fuller - Cleveland Heights OH
Joseph Szasz - Chesterland OH
Frank T. Robb - Silver Spring MD
Kimberly M. Borges - Ellicott City MD
Maria Davis - Twinsburg OH

Assignee:

Amersham Life Science, Inc. - Cleveland OH

International Classification:

C12N 1563
C12N 1511
C12N 1555

US Classification:

4353201

Abstract:

Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS. : 1-8.

US Patent:

57190569, Feb 17, 1998

Filed:

Apr 24, 1996

Appl. No.:

8/636928

Inventors:

Shauna R. Brummet - Wadsworth OH
Frank T. Robb - Silver Spring MD
Kimberly M. Borges - Coventry CT
Kristine M. Hujer - Cleveland OH
Sally T. Domke - Parma OH

Assignee:

Amersham Life Science, Inc. - Cleveland OH

International Classification:

C12N 1563
C12N 1511
C12N 1555
C12N 916

US Classification:

4353201

Abstract:

Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS. : 1-9.

US Patent:

20150211037, Jul 30, 2015

Filed:

Jul 14, 2011

Appl. No.:

13/813154

Inventors:

Joel Edward Graham - Baltimore MD, US
Melinda E. Clark - San Francisco CA, US
Frank Thomson Robb - Gaithersburg MD, US
Douglas S. Clark - Orinda CA, US
Harvey W. Blanch - San Francisco CA, US

Assignee:

University of Maryland, Baltimore - Baltimore MD
The Regents of the University of California - Oakland CA

International Classification:

C12P 19/14
C13K 1/02
C12N 9/42
C12P 19/02

Abstract:

Hydrolysis and degradation of cellulose-containing biomass by use of a polypeptide having cellulase activity is provided. Also provided are polypeptides having cellulase activity, such as archaeal cellulases, polynucleotides encoding the polypeptides, and compositions containing the polypeptides, and methods of use thereof.