Fred Levine

US Patent:

6448045, Sep 10, 2002

Filed:

Mar 10, 2000

Appl. No.:

09/522376

Inventors:

Fred Levine - Del Mar CA
Dominique Dufayet - San Diego CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12P 2102

US Classification:

435 701, 435455

Abstract:

The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are in contact with other cells in the culture, thereby inducing insulin gene expression in the cells. The invention also provides high throughput screening methods for modulators of -cell function, stable cultures of cells made by the methods of the invention, and methods of treating a human subject using the methods of the invention.

US Patent:

6528309, Mar 4, 2003

Filed:

Mar 19, 2001

Appl. No.:

09/812042

Inventors:

Fred Levine - Del Mar CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 506

US Classification:

435374, 435404, 435366

Abstract:

The present invention provides methods and compositions for the protection and storage of cells. In particular, the present invention provides methods and compositions for the vacuum-mediated desiccation protection of mammalian cells. In particularly preferred embodiments, cells are treated with a carbohydrate (e. g. , a disaccharide) prior to vacuum-mediated desiccation.

US Patent:

6884585, Apr 26, 2005

Filed:

May 30, 2002

Appl. No.:

10/160336

Inventors:

Fred Levine - Del Mar CA, US
Dominique Dufayet - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q001/68

US Classification:

435 6, 435 71, 435325, 435366, 435440, 435455

Abstract:

The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are in contact with other cells in the culture, thereby inducing insulin gene expression in the cells. The invention also provides high throughput screening methods for modulators of β-cell function, stable cultures of cells made by the methods of the invention, and methods of treating a human subject using the methods of the invention.

US Patent:

6911324, Jun 28, 2005

Filed:

Oct 18, 2001

Appl. No.:

10/041845

Inventors:

Fred Levine - Del Mar CA, US
Dominique Gouty - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12P021/00
C12N005/00

US Classification:

435 701, 435 41, 435 704, 435440, 435455, 435325, 435366

Abstract:

The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene and a NeuroD/BETA2 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are in contact with other cells in the culture, thereby inducing insulin gene expression in the cells. The invention also provides high throughput screening methods for modulators of β-cell function, stable cultures of cells made by the methods of the invention, and methods of treating a human subject using the methods of the invention.

US Patent:

7276352, Oct 2, 2007

Filed:

Jan 19, 2005

Appl. No.:

11/039523

Inventors:

Fred Levine - Del Mar CA, US
Dominique Gouty - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12P 21/00
C12N 5/08
C12N 15/09
C12N 15/87
C12N 5/06

US Classification:

435 41, 435 703, 435375, 435455, 435462, 435467

Abstract:

The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are in contact with other cells in the culture, thereby inducing insulin gene expression in the ceils. The invention also provides methods of treating a diabetic human subject using the methods of the invention.

US Patent:

7776593, Aug 17, 2010

Filed:

Aug 7, 2007

Appl. No.:

11/835391

Inventors:

Fred Levine - Del Mar CA, US
Bjoern Tyrberg - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 5/08
C12N 5/06
C12Q 1/68
C07K 16/00
C07H 21/04

US Classification:

435370, 435 6, 435325, 435363, 435366, 5303879, 536 235

Abstract:

The present invention provides methods of identifying endocrine stem and progenitor cells.

US Patent:

8168391, May 1, 2012

Filed:

Nov 7, 2008

Appl. No.:

12/267507

Inventors:

Fred Levine - Del Mar CA, US
Mark Mercola - Del Mar CA, US

Assignee:

Burnham Institute for Medical Research - La Jolla CA
The Regents of the University of California - Oakland CA

International Classification:

C12Q 1/68
G01N 33/53
G01N 33/567
C12N 15/00

US Classification:

435 613, 435 71, 435 721, 4353201

Abstract:

The present invention provides screening methods for the detection of agents that affect various aspects of β-cell biology, particularly insulin gene expression. Screening methods are also provided for detection of agents that affect β-cell differentiation from progenitor cells. Additionally, agents identified using such methods are provided and are useful for increasing insulin gene expression and reducing lipotoxicity.

US Patent:

20030202967, Oct 30, 2003

Filed:

Mar 27, 2003

Appl. No.:

10/402194

Inventors:

Fred Levine - Del Mar CA, US
Sijian Wang - Dallas TX, US
Gillian Beattie - Poway CA, US
Alberto Hayek - La Jolla CA, US

International Classification:

A61K048/00
C12N015/867
C12N005/08

US Classification:

424/093210, 435/456000, 435/366000, 435/320100

Abstract:

This invention relates to cell lines, particularly mammalian cell lines, established by transforming the cells with vectors, preferably retroviral vectors, containing two or more oncogenes under the control of one or more inducible promoters and/or genetic elements. Also within the scope of the invention are human cell lines with extended in vitro lifespan, transformed by vectors containing one or more oncogenes under the control of one or more, preferably exogenous, inducible promoters and/or genetic elements. The vectors may additionally contain gene(s) encoding for desired gene product(s). Also disclosed are insulin producing human pancreatic cell lines useful for transplantation into human diabetic patients.