Fred H Stevens

US Patent:

6485943, Nov 26, 2002

Filed:

Mar 22, 1999

Appl. No.:

09/274163

Inventors:

Fred J. Stevens - Naperville IL
Priscilla Wilkins Stevens - Evanston IL
Rosemarie Raffen - Elmhurst IL
Marianne Schiffer - Downers Grove IL

Assignee:

The University of Chicago - Chicago IL

International Classification:

C12N 1500

US Classification:

435 696, 5303871, 530350

Abstract:

A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.

US Patent:

6489120, Dec 3, 2002

Filed:

Mar 28, 2000

Appl. No.:

09/536444

Inventors:

Fred J. Stevens - Naperville IL

Assignee:

The United States of America as represented by the United States Department of Energy - Washington DC

International Classification:

G10N 3353

US Classification:

435 71, 435 6, 435 911, 435 912, 4352831, 4352852, 4352872, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

A method and system for detecting a target antigen using massively parallel immunoassay technology. In this system, high affinity antibodies of the antigen are covalently linked to small beads or particles. The beads are exposed to a solution containing DNA-oligomer-mimics of the antigen. The mimics which are reactive with the covalently attached antibody or antibodies will bind to the appropriate antibody molecule on the bead. The particles or beads are then washed to remove any unbound DNA-oligomer-mimics and are then immobilized or trapped. The bead-antibody complexes are then exposed to a test solution which may contain the targeted antigens. If the antigen is present it will replace the mimic since it has a greater affinity for the respective antibody. The particles are then removed from the solution leaving a residual solution. This residual solution is applied a DNA chip containing many samples of complimentary DNA.

US Patent:

6878521, Apr 12, 2005

Filed:

Nov 13, 2000

Appl. No.:

09/712819

Inventors:

Fred J. Stevens - Naperville IL, US
Yair Argon - Flossmoor IL, US
David P. Davis - Chicago IL, US
Rosemarie Raffen - Elmhurst IL, US

Assignee:

The University of Chicago - Chicago IL

International Classification:

G01N033/53
G01N033/576
A61K038/08

US Classification:

435 71, 435 721, 435326, 530329

Abstract:

A method of minimizing the aggregation tendencies of an amyloid forming protein is provided comprising identifying a portion of the protein that is critical to amyloid formation; and inserting a peptide at the portion. The invention also provides a peptide for insertion in an intact human kappa-IV light chain variable domain, the peptide comprising the following amino acid sequence Phe-Thr-Leu-Thr-Ile-Ser-Ser(SEQ ID NO. 1) wherein the subscript numbers are the residue location points in the domain. A method for preventing amyloid formation in human kappa-IV light chain variable domain is also provided, the method comprising inserting the peptide Phe-Thr-Leu-Thr-Ile-Ser-Ser(SEQ ID NO. 1) into the domain, wherein the subscript numbers indicate the residue location on the domain.

US Patent:

7659083, Feb 9, 2010

Filed:

Aug 27, 2008

Appl. No.:

12/199683

Inventors:

Fred J. Stevens - Naperville IL, US
Marianne Schiffer - Downers Grove IL, US
W. Carey Hanly - Chicago IL, US
Sandra L. Tollaksen - Montgomery IL, US

Assignee:

Uchicago Argonne, LLC - Chicago IL

International Classification:

G01N 33/00

US Classification:

435 721, 435 71, 435 794, 435 7021, 435328, 436501, 436512, 436518, 530300, 530350, 4241301

Abstract:

A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex.

US Patent:

20060127949, Jun 15, 2006

Filed:

Sep 23, 2005

Appl. No.:

11/233913

Inventors:

Fred Stevens - Naperville IL, US
Marianne Schiffer - Downers Grove IL, US
W. Hanly - Chicago IL, US
Sandra Tollaksen - Montgomery IL, US

International Classification:

G01N 33/53
C07K 16/44

US Classification:

435007100, 530387300

Abstract:

A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex

US Patent:

20080248050, Oct 9, 2008

Filed:

Jul 2, 2007

Appl. No.:

11/824726

Inventors:

Fred J. Stevens - Naperville IL, US

International Classification:

A61K 39/395
C07K 16/18
A61P 37/00

US Classification:

4241781, 5303871, 5303911

Abstract:

A meta-specific vaccine particle is provided. Also provided is a method for inducing an immune response in a human () or non-human previously exposed to the meta-specific vaccine, and subsequently infected with a pathogen.

US Patent:

20110130324, Jun 2, 2011

Filed:

May 29, 2009

Appl. No.:

13/002013

Inventors:

Fred J. Stevens - Naperville IL, US

Assignee:

UCHICAGO ARGONNE, LLC - Chicago IL

International Classification:

A61K 38/00
C07K 2/00
C07K 16/00
A61K 38/02
C40B 50/06
A61P 7/00
A61P 35/00
A61P 19/02
A61P 17/06
A61P 37/06

US Classification:

514 11, 530350, 5303871, 506 7, 506 26

Abstract:

Methods and compositions to control the stability of proteins with special emphasis on antibodies and proteins with antibody-like structures, e.g., having an “immunoglobulin-like” fold, are described. Controlling the stability facilities different applications for a protein with the same function, but different stability.

US Patent:

51338440, Jul 28, 1992

Filed:

Mar 15, 1990

Appl. No.:

7/494074

Inventors:

Fred J. Stevens - Naperville IL

Assignee:

United States Department of Energy - Washington DC

International Classification:

G01N 2726
B01D 5702
B03C 502

US Classification:

2041801

Abstract:

A novel method of electric field flow fractionation for separating solute molecules from a carrier solution is disclosed. The method of the invention utilizes an electric field that is periodically reversed in polarity, in a time-dependent, wave-like manner. The parameters of the waveform, including amplitude, frequency and wave shape may be varied to optimize separation of solute species. The waveform may further include discontinuities to enhance separation.