Gary Cunningham

US Patent:

20180284099, Oct 4, 2018

Filed:

Mar 23, 2018

Appl. No.:

15/933834

Inventors:

- College Park MD, US
- Washington DC, US
- Bethesda MD, US
Marshall Lynn Summar - Washington DC, US
Gary Cunningham - Washington DC, US
Anton Simeonov - Bethesda MD, US
Juan Marugan - Gaithersburg MD, US

Assignee:

University of Maryland, College Park - College Park MD
Children's National Medical Center - Washington DC
The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services - Bethesda MD

International Classification:

G01N 33/49
G01N 33/68
G01N 27/327
G01N 33/84
G01N 21/78

Abstract:

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

US Patent:

20180143210, May 24, 2018

Filed:

Apr 27, 2016

Appl. No.:

15/570269

Inventors:

- College Park MD, US
- Washington DC, US
Peter Kofinas - North Bethesda MD, US
Gary Cunningham - Washington DC, US

Assignee:

University of Maryland, College Park - College Park MD
CHILDREN'S NATIONAL MEDICAL CENTER - Washington DC

International Classification:

G01N 33/84
B01L 3/00
G01N 21/78
A61K 31/216
A61P 3/00

Abstract:

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

US Patent:

20170106054, Apr 20, 2017

Filed:

Jul 15, 2016

Appl. No.:

15/212009

Inventors:

- Washington DC, US
Gary Cunningham - Washington DC, US
Dione T. Kobayashi - Boston MA, US
James G. McArthur - Concord MA, US

International Classification:

A61K 38/44
A61K 45/06
A61K 9/48
A61K 9/00

Abstract:

The present invention provides compositions and methods of treating hyperphenylalaninemia (e.g., phenylketonuria) in a subject in need thereof comprising administering to the subject an effective amount of a phenylalanine dehydrogenase (PheDH) polypeptide. The present invention also provides pharmaceutical formulations comprising PheDH for lowering the phenylalanine concentration in the subject (e.g., in the intestines and/or blood).

US Patent:

20160231310, Aug 11, 2016

Filed:

Sep 2, 2014

Appl. No.:

14/915602

Inventors:

- College Park MD, US
- Washington DC, US
- Bethesda MD, US
Marshall Lynn SUMMAR - Washington DC, US
Gary CUNNINGHAM - Washington DC, US
Anton SIMEONOV - Bethesda MD, US
Juan MARUGAN - Gaithersburg MD, US

International Classification:

G01N 33/49
G01N 33/68
G01N 27/327

Abstract:

The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

US Patent:

20160168613, Jun 16, 2016

Filed:

Oct 17, 2013

Appl. No.:

14/436844

Inventors:

- College Park MD, US
- Washington DC, US
- Bethesda MD, US
Marshall Lynn Summar - Washington DC, US
Gary Cunningham - Washington DC, US
Anton Simeonov - Bethesda MD, US
Juan Marugan - Gaithersburg MD, US

International Classification:

C12Q 1/00
C12N 11/04
G01N 27/327
G01N 33/68

Abstract:

The present invention relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reagentless system comprising an electrode modified by hydrogel that comprises at least one enzyme that oxidizes at least one substrate that is at least one amino acid. In some embodiments, the biosensor comprises a hydrogel comprising alginate. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the hydrogel.