Gary F Gerard

US Patent:

6518019, Feb 11, 2003

Filed:

Feb 5, 1999

Appl. No.:

09/245026

Inventors:

Gary F. Gerard - Frederick MD
Michael D. Smith - Rockville MD
Deb K. Chatterjee - North Potomac MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912

Abstract:

The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures about about 55Â C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs ( and/or subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be RTs, RTs, or RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods.

US Patent:

6589768, Jul 8, 2003

Filed:

Dec 24, 1998

Appl. No.:

09/220329

Inventors:

Michael Leslie Kotewicz - Columbia MD
Gary Floyd Gerard - Frederick MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 912

US Classification:

435194, 435 911, 435 912, 4352523, 4353201, 435471

Abstract:

The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

US Patent:

6610522, Aug 26, 2003

Filed:

Dec 24, 1998

Appl. No.:

09/220330

Inventors:

Michael Leslie Kotewicz - Columbia MD
Gary Floyd Gerard - Frederick MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 912

US Classification:

435194, 435 691, 435 911, 435 912, 43525233, 4352523, 4353201, 435471, 536 232

Abstract:

The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

US Patent:

6830902, Dec 14, 2004

Filed:

Jun 30, 2000

Appl. No.:

09/608066

Inventors:

Mekbib Astatke - Gaithersburg MD
Deb K. Chatterjee - North Potomac MD
Gary F. Gerard - Frederick MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P 1934

US Classification:

435 911, 435 71

Abstract:

The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e. g. , at ambient temperatures). Thus, in a preferred aspect, the invention relates to âhot startâ synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention.

US Patent:

6835561, Dec 28, 2004

Filed:

Feb 5, 1999

Appl. No.:

09/245025

Inventors:

Gary F. Gerard - Frederick MD
Michael D. Smith - Rockville MD
Deb K. Chatterjee - North Potomac MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 912

US Classification:

435194, 435193

Abstract:

The invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity and to methods of producing, amplifying or sequencing nucleic acid molecules using these compositions or polypeptides, particularly at temperatures above about 55Â C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and use of such nucleic acid molecules to produce desired polypeptide. The invention also relates to methods for producing Avian Sarcoma-Leukosis Virus (ASLV) RT subunits, in particular, Avian Myeloblastosis Virus (AMV) RTs, to isolated nucleic acid molecules encoding ASLV RT subunits, and to ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant RT holoenzymes, particularly ASLV RTs, methods for producing these RTs and to RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, and ASLV RTs of the invention.

US Patent:

6964861, Nov 15, 2005

Filed:

Nov 12, 1999

Appl. No.:

09/438358

Inventors:

Gary F. Gerard - Frederick MD, US
Elizabeth Flynn - Columbia MD, US
A-Li W. Hu - Kensington MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P019/34
C12N015/64
C12N015/00
C12Q001/68
C07H021/04

US Classification:

435 911, 435 914, 435 9142, 435 691, 4353201, 4352523, 435 6, 536 231, 536 242

Abstract:

The present invention relates generally to compositions and methods for enhancing recombinational cloning of nucleic acid molecules. In particular, the invention relates to compositions comprising one or more ribosomal proteins and one or more additional protein components required for recombinational cloning. More particularly, the invention relates to such compositions wherein the ribosomal proteins are one or more ribosomal proteins, still more particularly wherein the ribosomal proteins are selected from the group of ribosomal proteins consisting of S10, S14, S15, S16, S17, S18, S19, S20, S21, L20, L21, and L23 through L34, and most particularly S20, L27, and S15. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments. The invention also relates to isolated nucleic acid molecules produced by the methods of the invention, to vectors comprising such nucleic acid molecules, and to host cells comprising such nucleic acid molecules and vectors.

US Patent:

6989259, Jan 24, 2006

Filed:

Apr 22, 1998

Appl. No.:

09/064057

Inventors:

Gary F. Gerard - Frederick MD, US
Michael D. Smith - Rockville MD, US
Deb K. Chatterjee - North Potomac MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 9/12

US Classification:

435194, 435325, 4352541, 4353201, 536 232

Abstract:

The invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity and to methods of producing, amplifying or sequencing nucleic acid molecules using these compositions or polypeptides, particularly at temperatures above about 55 C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and use of such nucleic acid molecules to produce desired polypeptide. The invention also relates to methods for producing Avian Sarcoma-Leukosis Virus (ASLV) RT subunits, in particular, Avian Myeloblastosis Virus (AMV) RTs, to isolated nucleic acid molecules encoding ASLV RT subunits, and to ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant RT holoenzymes, particularly ASLV RTs, methods for producing these RTs and to RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, and ASLV RTs of the invention.

US Patent:

7078208, Jul 18, 2006

Filed:

May 1, 2001

Appl. No.:

09/845157

Inventors:

Michael D. Smith - Rockville MD, US
Robert Jason Potter - Frederick MD, US
Gulshan Dhariwal - Potomac MD, US
Gary F. Gerard - Frederick MD, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 9/12

US Classification:

435194, 435 911, 435 912

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.