Geoffrey R Hudson

US Patent:

6369207, Apr 9, 2002

Filed:

Sep 14, 1999

Appl. No.:

09/396001

Inventors:

Randall L. Dimond - Madison WI
Steven J. Ekenberg - Mt. Horeb WI
James R. Hartnett - Madison WI
Geoffrey R. Hudson - Madison WI
Leopoldo G. Mendoza - Madison WI
Katharine M. Miller - Verona WI
John E. Monahan - Walpole MA
Christopher L. Jones - Madison WI
Mark A. Maffitt - Madison WI
Richard A. Martinelli - Brighton MA
Edward E. Pahuski - Marshall WI
James W. Schumm - Madison WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C07H 1900

US Classification:

536 221, 536 231, 536 243, 536 2433

Abstract:

The present invention entails methods, and kits for carrying them out, based on the discovery that an RNA replicase, such as Q replicase, has DNA-dependent RNA polymerase (âDDRPâ) activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2â-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase. The discovery of this DDRP activity provides methods of the invention for nucleic acid amplification wherein a nucleic acid, with a DNA segment with the sequence of an RNA that is autocatalytically replicatable by an RNA replicase, is provided as a substrate for the replicase. The replicase catalyzes synthesis, from the DNA segment, of the RNA, which the replicase then autocatalytically replicates. The invention entails use of the amplification methods in detecting nucleic acid analytes, as in nucleic acid probe hybridization assays.

US Patent:

6677140, Jan 13, 2004

Filed:

Feb 7, 2002

Appl. No.:

10/071057

Inventors:

Randall L. Dimond - Madison WI
Steven J. Ekenberg - Mt. Horeb WI
James R. Hartnett - Madison WI
Geoffrey R. Hudson - Madison WI
Leopoldo G. Mendoza - Madison WI
Katharine M. Miller - Verona WI
John E. Monahan - Walpole MA
Christopher L. Jones - Madison WI
Mark A. Maffitt - Madison WI
Richard A. Martinelli - Brighton MA
Edward E. Pahuski - Marshall WI
James W. Schumm - Madison WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C12P 1934

US Classification:

435 911, 435 6, 435 912, 536 221, 536 231, 536 243, 536 2433

Abstract:

The present invention entails methods and kits for carrying them out based on the discovery that an RNA replicase, such as Q replicase, has DNA-dependent RNA polymerase (âDDRPâ) activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2â-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase.

US Patent:

60905891, Jul 18, 2000

Filed:

Jun 6, 1995

Appl. No.:

8/480041

Inventors:

Randall L. Dimond - Madison WI
Steven J. Ekenberg - Mt. Horeb WI
James R. Hartnett - Madison WI
Geoffrey R. Hudson - Madison WI
Leopoldo G. Mendoza - Conroe TX
Katharine M. Miller - Verona WI
John E. Monahan - Walpole MA
Christopher L. Jones - Madison WI
Mark A. Maffitt - Madison WI
Richard A. Martinelli - Brighton MA
Edward E. Pahuski - Marshall WI
James W. Schumm - Madison WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168
C12P 1934
C07H 1900
C07H 2104

US Classification:

435 911

Abstract:

The present invention entails methods, and kits for carrying them out, based on the discovery that an RNA replicase, such as Q. beta. replicase, has DNA-dependent RNA polymerase ("DDRP") activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2'-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase. The discovery of this DDRP activity provides methods of the invention for nucleic acid amplification wherein a nucleic acid, with a DNA segment with the sequence of an RNA that is autocatalytically replicatable by an RNA replicase, is provided as a substrate for the replicase. Assays of the invention include those wherein a nucleic acid analyte is hybridized with one or more nucleic acid probes, which include or are processed to generate a DNA segment which is amplifiable through production from the segment, catalyzed by the DDRP activity of an RNA replicase, of an autocatalytically replicatable RNA, which is autocatalytically replicated to provide an abundance of readily detectable reporter molecules.

US Patent:

61000248, Aug 8, 2000

Filed:

Feb 8, 1991

Appl. No.:

7/652888

Inventors:

Geoffrey R. Hudson - Madison WI
James W. Schumm - Madison WI
Randall L. Dimond - Madison WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 168
C07H 2104

US Classification:

435 6

Abstract:

The present invention provides a novel, single-stranded DNA probe which comprises an anti-target segment, a strand of a promoter, and a reporter segment, arranged so that a target segment, which has a 3'-hydroxyl at its terminus, can prime DNA polymerase-catalyzed extension of the target segment along the probe as template, when the target segment is hybridized to the anti-target segment of the probe, to provide an extension product from which transcripts, with the sequence complementary to that of the reporter segment of the probe, can be made by transcription from the promoter corresponding to the promoter segment of the probe. The transcripts, optionally after further amplification or other processing, can be detected. In one embodiment of the invention, the transcripts will be autocatalytically replicatable by an RNA replicase such as Q. beta. replicase.