George M Janini

US Patent:

20060057556, Mar 16, 2006

Filed:

Oct 20, 2003

Appl. No.:

10/529967

Inventors:

George Janini - Rockville MD, US
Haleem Issaq - Frederick MD, US
Timothy Veenstra - Jefferson MD, US
Thomas Conrads - Frederick MD, US
Kenneth Wilkens - Brentwood TN, US

Assignee:

The Government of the United States of America Department of Health and Human Services - Rockville MD

International Classification:

C12M 1/34
C12Q 1/00

US Classification:

435004000, 435287100

Abstract:

Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are also disclosed. Apparatus and methods for conveying analyte ions from the capillaries into analytical instruments, such as a mass spectrometer, are also disclosed. The disclosed contiguous capillaries may be used to carryout electrophoresis separation and electrospray ionization of analytes. Methods for obtaining the mass spectra of macromolecular analytes at concentrations lower than previously possibly are provided using the apparatus and procedures described herein.