George A Walker

US Patent:

20030104430, Jun 5, 2003

Filed:

Jul 15, 2002

Appl. No.:

10/197185

Inventors:

Michael Nerenberg - La Jolla CA, US
Carl Edman - San Diego CA, US
Catherine Spargo - Apex. NC, US
George Walker - Chapel Hill NC, US

International Classification:

C12Q001/68
C12P019/34
C12M001/34
H01L021/00

US Classification:

435/006000, 435/091200, 435/287200, 438/001000

Abstract:

Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelectronic microchip, the addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequence specific reporter, or by a method of sequence specific capture with universal reporter. The label may be detected by fluorescence, chemiluminescence, elecrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic acids may be amplified while either in direct contact with microchip components or in solution above the microchip array.

US Patent:

20060110754, May 25, 2006

Filed:

Oct 14, 2005

Appl. No.:

11/251229

Inventors:

Michael Nerenberg - La Jolla CA, US
Carl Edman - San Diego CA, US
Catherine Spargo - Apex NC, US
George Walker - Chapel Hill NC, US

International Classification:

C12Q 1/68
C12P 19/34
C12M 1/34

US Classification:

435006000, 435091200, 435287200

Abstract:

Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelectronic microchip, the addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequence specific reporter, or by a method of sequence specific capture with universal reporter. The label may be detected by fluorescence, chemiluminescence, elecrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic acids may be amplified while either in direct contact with microchip components or in solution above the microchip array.

US Patent:

20070202536, Aug 30, 2007

Filed:

Aug 2, 2006

Appl. No.:

11/497919

Inventors:

Douglas Yamanishi - Carlsbad CA, US
Paul Hujsak - San Diego CA, US
Sara Snyder - San Francisco CA, US
George Walker - San Diego CA, US
Junquan Xu - San Diego CA, US
Mingxian Huang - San Diego CA, US
Guoliang Tao - San Diego CA, US
Lei Wu - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Joe Ouyang - San Diego CA, US
Charina Schmitigal - San Diego CA, US
Samantha O'Neill - San Diego CA, US
Jing Cheng - San Diego CA, US
Jia Xu - San Diego CA, US

International Classification:

C12N 5/06
G01N 33/53

US Classification:

435007100, 435378000

Abstract:

The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.

US Patent:

52444635, Sep 14, 1993

Filed:

Dec 6, 1991

Appl. No.:

7/804828

Inventors:

Edward T. Cordner - Oceanside CA
George T. Walker - San Diego CA
Howard S. Barr - Escondido CA
Farid Khadem - San Diego CA

Assignee:

Block Medical, Inc. - Carlsbad CA

International Classification:

A61M 3700

US Classification:

604131

Abstract:

An infusion pump system, comprises disposable tubing having an elastic segment and one-way check valves at each end of the segment for conveying intravenous fluid from a source to a patient, a compact, portable housing having an open receptacle for removably receiving a segment of the disposable tubing, a reciprocating ram mounted in the housing for periodically engaging and compressing the segment of the disposable tubing for pumping fluid therethrough, a motor mounted in the housing for driving the ram upon energization thereof, a control system mounted in the housing for receiving input signals and energizing the motor so that the fluid is pumped through the disposable tubing in accordance with pre-selected delivery rate parameters.