George Terrance Walker

US Patent:

RE39885, Oct 16, 2007

Filed:

May 20, 1998

Appl. No.:

09/082247

Inventors:

James G. Nadeau - Ellicott City MD, US
George T. Walker - Chapel Hill NC, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.

US Patent:

7531316, May 12, 2009

Filed:

May 27, 2004

Appl. No.:

10/557540

Inventors:

Yun-Fu Hu - Research Triangle Park NC, US
George T. Walker - Research Triangle Park NC, US

Assignee:

Glaxo Group Limited - Greenford, Middlesex

International Classification:

C12Q 1/34

US Classification:

435 18, 435198

Abstract:

This invention relates to a method for determining the activity of Lp-PLA2 in a plurality of samples from animals. The invention also relates to a kit for determining Lp-PLA2 activity in a plurality of samples.

US Patent:

7741020, Jun 22, 2010

Filed:

Apr 14, 2005

Appl. No.:

11/106239

Inventors:

Yaping Shou - Research Triangle Park NC, US
George T. Walker - Research Triangle Park NC, US

Assignee:

Glaxo Group Limited - Greenford, Middlesex

International Classification:

C12Q 1/00

US Classification:

435 4

Abstract:

This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

US Patent:

8609357, Dec 17, 2013

Filed:

Jul 2, 2012

Appl. No.:

13/540571

Inventors:

Yaping Shou - Research Triangle Park NC, US
George T. Walker - Research Triangle Park NC, US

Assignee:

Glaxo Group Limited - Greenford, Middlesex

International Classification:

C12Q 1/44
C12Q 1/00

US Classification:

435 19, 435 4

Abstract:

This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

US Patent:

20030048108, Mar 13, 2003

Filed:

May 14, 2002

Appl. No.:

10/145661

Inventors:

Brian Beaman - Apex NC, US
Fuad Doany - Katonah NY, US
Keith Fogel - Mohegan Lake NY, US
James Hedrick - Pleasanton CA, US
Paul Lauro - Brewster NY, US
Maurics Norcott - San Jose CA, US
John Ritsko - Mount Kisco NY, US
Sang-Hyon Paek - Yongin-shi, KR
Leathen Shi - Yorktown Heights NY, US
Da-Yuan Shih - Poughkeepsie NY, US
George Walker - New York NY, US

International Classification:

G01R031/02

US Classification:

324/754000

Abstract:

The present invention is directed to a structure comprising a substrate having a surface; a plurality of elongated electrical conductors extending away from the surface; each of said elongated electrical conductors having a first end affixed to the surface and a second end projecting away from the surface; there being a plurality of second ends; and a means for positioning and maintaining the plurality of the second ends in substantially fixed positions with respect to each other. The structure is useful as a probe for testing and burning in integrated circuit chips at the wafer level.

US Patent:

20030104430, Jun 5, 2003

Filed:

Jul 15, 2002

Appl. No.:

10/197185

Inventors:

Michael Nerenberg - La Jolla CA, US
Carl Edman - San Diego CA, US
Catherine Spargo - Apex. NC, US
George Walker - Chapel Hill NC, US

International Classification:

C12Q001/68
C12P019/34
C12M001/34
H01L021/00

US Classification:

435/006000, 435/091200, 435/287200, 438/001000

Abstract:

Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelectronic microchip, the addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequence specific reporter, or by a method of sequence specific capture with universal reporter. The label may be detected by fluorescence, chemiluminescence, elecrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic acids may be amplified while either in direct contact with microchip components or in solution above the microchip array.

US Patent:

20060110754, May 25, 2006

Filed:

Oct 14, 2005

Appl. No.:

11/251229

Inventors:

Michael Nerenberg - La Jolla CA, US
Carl Edman - San Diego CA, US
Catherine Spargo - Apex NC, US
George Walker - Chapel Hill NC, US

International Classification:

C12Q 1/68
C12P 19/34
C12M 1/34

US Classification:

435006000, 435091200, 435287200

Abstract:

Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelectronic microchip, the addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequence specific reporter, or by a method of sequence specific capture with universal reporter. The label may be detected by fluorescence, chemiluminescence, elecrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic acids may be amplified while either in direct contact with microchip components or in solution above the microchip array.

US Patent:

20070166777, Jul 19, 2007

Filed:

Apr 14, 2005

Appl. No.:

10/599914

Inventors:

Yaping Shou - Research Triangle Park NC, US
George Walker - Research Triangle Park NC, US

International Classification:

C12Q 1/48
C12Q 1/34

US Classification:

435015000, 435018000

Abstract:

This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.