Gina L Lipscomb

US Patent:

20120135411, May 31, 2012

Filed:

Sep 29, 2011

Appl. No.:

13/248377

Inventors:

Gina L. Lipscomb - Athens GA, US
Joel Andrew Farkas - Athens GA, US
Michael W.W. Adams - Athens GA, US
Janet Westpheling - Athens GA, US

International Classification:

C12Q 1/68
C12N 1/00
C12N 15/74

US Classification:

435 612, 435 61, 435471, 435243, 4353201

Abstract:

Provided herein are methods for transforming a with a polynucleotide. In one embodiment, the method includes contacting a with a polynucleotide under conditions suitable for uptake of the polynucleotide by the , and identifying transformants at a frequency of, for instance, at least 10transformants per microgram DNA. Also provided are isolated having the characteristics of COM1, and plasmids that include an origin of replication that functions in a . The plasmid is stable in a recipient without selection for more than 100 generations and is structurally unchanged after replication in for more than 100 generations.

US Patent:

20150211030, Jul 30, 2015

Filed:

Sep 6, 2013

Appl. No.:

14/426290

Inventors:

- Athens GA, US
- Raleigh NC, US
Aaron B. Hawkins - Durham NC, US
Angeli Lal Menon - Athens GA, US
Gina Pries Lipscomb - Athens GA, US
Gerrit Jan Schut - Athens GA, US

Assignee:

North Carolina State University - Raleigh NC

International Classification:

C12P 7/42
C12N 9/90
C12N 9/88
C12N 9/00
C12N 9/02

Abstract:

Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.

US Patent:

20140248687, Sep 4, 2014

Filed:

Nov 2, 2012

Appl. No.:

14/355362

Inventors:

- Athens GA, US
- Raleigh NC, US
Mirko Basen - Athens GA, US
Gina Pires Lipscomb - Athens GA, US
Angeli Menon - Athens GA, US

Assignee:

University of Georgia Research Foundation, Inc. - Athens GA
North Carolina State University - Raleigh NC

International Classification:

C12N 15/74

US Classification:

4352523

Abstract:

Provided herein are genetically engineered archaea. A genetically engineered archaea includes a heterologous polynucleotide that has a promoter operably linked to a coding region, where the coding region encodes a polypeptide having optimal activity below the optimum growth temperature (T) of the genetically engineered archaeon. Also provided herein are methods for using genetically engineered archaea and cell-free extracts of such genetically engineered archaea. In one embodiment, the methods include culturing a genetically engineered archaeon at a temperature that is at least 20 C. below the Tof the genetically engineered archaeon, such that the activity in the genetically engineered archaeon of a polypeptide encoded by the coding region is increased compared to the activity in the genetically engineered archaeon of the polypeptide during growth at a second temperature that is at or near the Tof the genetically engineered archaeon.