Gordon M Mills

US Patent:

8211646, Jul 3, 2012

Filed:

Nov 16, 2009

Appl. No.:

12/619345

Inventors:

Meera Nanjundan - Tampa FL, US
Gordon Mills - Houston TX, US
Dawn Smith - Polk City FL, US

Assignee:

University of South Florida - Tampa FL

International Classification:

C12Q 1/68

US Classification:

435 614

Abstract:

Genomic analysis of ovarian cancers demonstrated a regional chromosomal increase in expression and gene duplication. TGF-β stimulation indicated a link between SnoN RNA and TGF-β. In TIOSE, SnoN protein levels were reduced 15 min post TGF-β-stimulation, likely by proteosome-mediated degradation. SnoN inhibition decreased cell growth between 20 and 50% concurrent with increased p21 levels. Stable expression of SnoN led to growth arrest through induction of senescence. Collectively, these results implicate SnoN levels in multiple roles during ovarian carcinogenesis: promoting cellular proliferation in ovarian cancer cells and as a positive mediator of cell cycle arrest and senescence in non-transformed ovarian epithelial cells.