Gregory P Keyes

US Patent:

20150322107, Nov 12, 2015

Filed:

Jul 17, 2015

Appl. No.:

14/801897

Inventors:

- Ann Arbor MI, US
Levi Lynn Blazer - Ann Arbor MI, US
Fred Lawrence Ciske - Dexter MI, US
Gregory William Endres - Saline MI, US
Jeffrey Keith Johnson - Ann Arbor MI, US
Gregory Scott Keyes - Dexter MI, US
Ranjinder Singh Sidhu - Ann Arbor MI, US
Raymond C. Trievel - Ypsilanti MI, US
Margaret Lynn Collins - Canton MI, US

International Classification:

C07H 19/16
G01N 33/58

Abstract:

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

US Patent:

20150323542, Nov 12, 2015

Filed:

Jul 17, 2015

Appl. No.:

14/801902

Inventors:

- Ann Arbor MI, US
Levi Lynn Blazer - Ann Arbor MI, US
Fred Lawrence Ciske - Dexter MI, US
Gregory William Endres - Saline MI, US
Jeffrey Keith Johnson - Ann Arbor MI, US
Gregory Scott Keyes - Dexter MI, US
Ranjinder Singh Sidhu - Ann Arbor MI, US
Raymond C. Trievel - Ypsilanti MI, US
Margaret Lynn Collins - Canton MI, US

International Classification:

G01N 33/58
G01N 21/64
C07D 519/00
C07D 473/34
C07D 471/04

Abstract:

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.