Gregory Keyes

US Patent:

20120295283, Nov 22, 2012

Filed:

May 18, 2012

Appl. No.:

13/475618

Inventors:

Stephen Douglas Barrett - Hartland MI, US
Daniel Austin Bochar - Ann Arbor MI, US
Levi Lynn Blazer - Ann Arbor MI, US
Fred Lawrence Ciske - Dexter MI, US
Gregory William Endres - Saline MI, US
Jeffrey Keith Johnson - Ann Arbor MI, US
Gregory Scott Keyes - Dexter MI, US
Ranjinder Singh Sidhu - Ann Arbor MI, US
Raymond C. Trievel - Ypsilanti MI, US
Margaret Lynn Collins - Canton MI, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF MICHIGAN - Ann Arbor MI
CAYMAN CHEMICAL COMPANY, INCORPORATED - Ann Arbor MI

International Classification:

C07D 473/34
G01N 21/64

US Classification:

435 78, 436501, 544277

Abstract:

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.