Gregory W Payne

US Patent:

8562809, Oct 22, 2013

Filed:

Oct 2, 2009

Appl. No.:

13/122403

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
W. Lee Meyer - Baltimore MD, US

Assignee:

University of Maryland, College Park - College Park MD

International Classification:

C25D 9/02

US Classification:

205229, 205317

Abstract:

A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electrodepositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.

US Patent:

20080254104, Oct 16, 2008

Filed:

Mar 17, 2008

Appl. No.:

12/077173

Inventors:

Srinivasa R. Raghavan - Silver Spring MD, US
Gregory F. Payne - Cockeysville MD, US
Chao Zhu - McLean VA, US
Matthew B. Dowling - Washington DC, US

International Classification:

A61K 9/70
A61P 17/02

US Classification:

424445

Abstract:

The present invention provides a novel biomaterial which is a hybrid, self-assembling biopolymeric networked film that is functionalized through hydrophobic interactions with vesicles loaded with bioactive agents. The biomaterial compound is a polymeric network of hydrophobically modified chitosan scaffolds that is taken from solution and formed as a solid film. This solid state film is capable of hydrophobic interactions with the functionalized vesicles. The vesicles include one or more lamellar structures forming one or more nano-compartments that are capable of containing similar or alternative active moieties within. Use of the film results in a degradation of the chitosan scaffold thereby releasing the active moieties within the vesicles from the scaffold. Application of the current invention occurs through various delivery mechanisms and routes of administration as will be described herein.

US Patent:

20120103822, May 3, 2012

Filed:

Apr 27, 2010

Appl. No.:

13/266433

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
Xiaohua Yang - Ardmore OK, US

Assignee:

UNIVERSITY OF MARYLAND COLLEGE PARK - College Park MD

International Classification:

C25D 9/02
G01N 33/53
C12N 13/00

US Classification:

205317, 4351731, 435 71

Abstract:

Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.

US Patent:

20120252703, Oct 4, 2012

Filed:

Dec 2, 2011

Appl. No.:

13/310579

Inventors:

Matthew Dowling - Washington DC, US
Srinivasa R. Raghavan - Silver Spring MD, US
Neeraja Dashaputre - College Park MD, US
Douglas Stephen English - Derby KS, US
Vishal Javvaji - Chantilly VA, US
Gregory F. Payne - Hunt Valley MD, US
Philip R. DeShong - Silver Spring MD, US

Assignee:

University of Maryland,College Park Office of Technology and Commercialization - College Park MD

International Classification:

G01N 33/533
C25D 9/02
C25D 5/34
C40B 60/08

US Classification:

506 37, 422 69, 205317, 205211

Abstract:

A biosensor comprising an electrically conductive substrate coated with a modified chitosan biopolymer that has been electrodeposited on the substrate, wherein said modified chitosan biopolymer comprises at least one vesicle binding molecule. The biosensor is manufactured by a method where a modified chitosan biopolymer is electrodeposited on a substrate. The method is also used to manufacture a modified chitosan biopolymer film by electrodeposition of the chitosan on the substrate and later removing the film from the substrate after electrodeposition. The resulting film can be used in bandages to treat various types of wounds. The biosensor can also be used to detect various analytes in samples.

US Patent:

20170326169, Nov 16, 2017

Filed:

Feb 27, 2017

Appl. No.:

15/442911

Inventors:

- College Park MD, US
Srinivasa R. Raghavan - Silver Spring MD, US
Gregory F. Payne - Cockeysville MD, US
Chao Zhu - McLean VA, US

International Classification:

A61K 31/722
A61K 47/69
A61K 47/56
A61L 26/00
A61K 9/127
A61K 9/70
A61K 47/61
A61L 27/20
A61K 9/127

Abstract:

The present invention provides a novel biomaterial which is a hybrid, self-assembling biopolymeric networked film that is functionalized through hydrophobic interactions with vesicles loaded with bioactive agents. The biomaterial compound is a polymeric network of hydrophobically modified chitosan scaffolds that is taken from solution and formed as a solid film. This solid state film is capable of hydrophobic interactions with the functionalized vesicles. The vesicles include one or more lamellar structures forming one or more nano-compartments that are capable of containing similar or alternative active moieties within. Use of the film results in a degradation of the chitosan scaffold thereby releasing the active moieties within the vesicles from the scaffold. Application of the current invention occurs through various delivery mechanisms and routes of administration as will be described herein.

US Patent:

20160213704, Jul 28, 2016

Filed:

Jun 29, 2015

Appl. No.:

14/754600

Inventors:

- College Park MD, US
Srinivasa R. Raghavan - Silver Spring MD, US
Gregory F. Payne - Cockeysville MD, US
Chao Zhu - McLean VA, US

International Classification:

A61K 31/722

Abstract:

The present invention provides a novel biomaterial which is a hybrid, self-assembling biopolymeric networked film that is functionalized through hydrophobic interactions with vesicles loaded with bioactive agents. The biomaterial compound is a polymeric network of hydrophobically modified chitosan scaffolds that is taken from solution and formed as a solid film. This solid state film is capable of hydrophobic interactions with the functionalized vesicles. The vesicles include one or more lamellar structures forming one or more nano-compartments that are capable of containing similar or alternative active moieties within. Use of the film results in a degradation of the chitosan scaffold thereby releasing the active moieties within the vesicles from the scaffold. Application of the current invention occurs through various delivery mechanisms and routes of administration as will be described herein.

US Patent:

20140332410, Nov 13, 2014

Filed:

May 9, 2014

Appl. No.:

14/274643

Inventors:

- College Park MD, US
Reza Ghodssi - Potomac MD, US
Gregory F. Payne - Hunt Valley MD, US
Deanna L Kelly - York PA, US
Eunkyoung Kim - Woodstock MD, US
Thomas E Winkler - Greenbelt MD, US

Assignee:

University of Maryland - College Park MD

International Classification:

G01N 27/327
B01L 3/00

US Classification:

205782, 20440301

Abstract:

A lab on a chip device includes a whole blood inlet port and microchannels to transport a whole blood sample or plasma skimmed from the whole blood sample into a detection chamber that includes at least one 3-electrode set of a counter electrode, a working electrode and a reference electrode. The counter electrode, the working electrode and the reference electrode may present bare, unmodified surfaces that are disposed so that clozapine present in the whole blood sample is detected via a reduction-oxidation reaction. Alternatively, the working electrode surface may include catechol grafted to chitosan. A method of detecting analytes and biomarkers includes collecting a whole blood sample, loading the sample into a point-of-care testing (POCT) device that includes at least one working electrode; testing the sample for the occurrence of a redox reaction; and calculating the total oxidative charge when the working electrode is bare or modified as before.