Gretchen K Johnson

US Patent:

7129086, Oct 31, 2006

Filed:

Jun 4, 2002

Appl. No.:

10/162825

Inventors:

Beverly Torok-Storb - Seattle WA, US
Bryan A. Roecklein - Seattle WA, US
Gretchen Johnson - Issaquah WA, US

Assignee:

Fred Hutchinson Cancer Research Center - Seattle WA

International Classification:

C12N 5/00

US Classification:

435366, 435325, 435404, 435 701, 435372, 435405

Abstract:

Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1α, and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.

US Patent:

6103522, Aug 15, 2000

Filed:

May 31, 1995

Appl. No.:

8/455577

Inventors:

Beverly Torok-Storb - Seattle WA
Bryan A. Roecklein - Seattle WA
Gretchen Johnson - Issaquah WA

Assignee:

Fred Hutchinson Cancer Research Center - Seattle WA

International Classification:

C12N 500

US Classification:

435325

Abstract:

Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1. alpha. , and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.

US Patent:

58799400, Mar 9, 1999

Filed:

May 23, 1997

Appl. No.:

8/862232

Inventors:

Beverly Torok-Storb - Seattle WA
Bryan A. Roecklein - Seattle WA
Gretchen Johnson - Issaquah WA

Assignee:

Fred Hutchinson Cancer Research Center - Seattle WA

International Classification:

L12N 500

US Classification:

435404

Abstract:

Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1. alpha. , and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.