Guangrong Zhang

US Patent:

6632598, Oct 14, 2003

Filed:

Mar 11, 1994

Appl. No.:

08/212175

Inventors:

Guangrong Zhang - San Ramon CA
Cheng-Zhi Yu - Pleasant Hill CA
Sheng-Hui Su - San Ramon CA
Krishan L. Kalra - Danville CA
Ding Zhou - Pleasant Hill CA

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

C12Q 100

US Classification:

435 4, 435 405, 435267

Abstract:

Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

US Patent:

7070951, Jul 4, 2006

Filed:

Aug 29, 2003

Appl. No.:

10/652854

Inventors:

Guangrong Zhang - San Ramon CA, US
Cheng-Zhi Yu - Pleasant Hill CA, US
Sheng-Hui Su - San Ramon CA, US
Krishan L. Kalra - Danville CA, US
Ding Zhou - Pleasant Hill CA, US

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

G01N 1/30

US Classification:

435 4052, 435 405, 435975

Abstract:

Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

US Patent:

20060019332, Jan 26, 2006

Filed:

Jul 20, 2005

Appl. No.:

11/186392

Inventors:

Guangrong Zhang - San Ramon CA, US
Cheng-Zhi Yu - Pleasant Hill CA, US
Sheng-Hui Su - San Ramon CA, US
Krishan Kalra - Danville CA, US
Ding Zhou - Pleasant Hill CA, US

International Classification:

G01N 1/30
G01N 33/48
C23G 5/00

US Classification:

435040500, 252364000

Abstract:

Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.