Harold E Crews

US Patent:

6632676, Oct 14, 2003

Filed:

Sep 24, 1999

Appl. No.:

09/405547

Inventors:

Harold Richardson Crews - Coral Springs FL
Ted Sena - Naples FL

Assignee:

Clinical Diagnostic Solutions - Plantation FL

International Classification:

G01N 3100

US Classification:

436 18, 436 8, 436 10, 436 15, 436 17, 436 63, 436164, 436166, 436174, 436176, 436179, 435 2, 435 405

Abstract:

A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d. c. impedance volume, conductivity/opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements.

US Patent:

6869798, Mar 22, 2005

Filed:

Apr 17, 2003

Appl. No.:

10/418196

Inventors:

Harold Richardson Crews - Coral Springs FL, US
Michael N. Elliott - Fort Lauderdale FL, US

Assignee:

Clinical Diagnostics Solutions, Inc. - Plantation FL

International Classification:

G01N031/00
G01N033/48

US Classification:

436 10, 436 8, 436 17, 436 18, 436 63, 436164, 436166, 435 2, 435 29, 435 39, 2524081

Abstract:

A lytic reagent composition and the method of use for differential analysis of leukocytes using flow cytometry are disclosed. The lytic reagent composition includes a short chain alkyl oxyethanol, such as 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol, or 2-isopropoxyethanol, in a sufficient amount to preserve leukocytes; a non-lysing nonionic surfactant as a debris solublizer; and an inorganic buffer to maintain pH of the lytic reagent composition in a range from 9. 1 to 10. 7. The lytic reagent composition is used to lyse red blood cells and preserve leukocytes, as well as used as the sheath reagent for the focus flow measurement. The lytic reagent composition, when used on a flow cytometric analyzer with multiple angle light scatter and light absorbance measurements, enables differentiation of leukocytes into five subpopulations.

US Patent:

20020098589, Jul 25, 2002

Filed:

Jan 19, 2001

Appl. No.:

09/766372

Inventors:

Harold Crews - Coral Springs FL, US
James Carter II - Plantation FL, US
Michael Elliot - Fort Lauderdale FL, US

International Classification:

G01N033/48
G01N033/53
G01N021/75

US Classification:

436/010000, 436/063000, 436/805000, 436/808000, 436/166000

Abstract:

A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d.c. impedance volume, conductivity/opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements. Novel methods for use of the reagents with automated and semi-automated hematology analyzers are also provided.

US Patent:

20040241769, Dec 2, 2004

Filed:

Mar 2, 2004

Appl. No.:

10/791309

Inventors:

Harold Crews - Coral Springs FL, US
James Carter - Plantation FL, US
Michael Elliot - Fort Lauderdale FL, US

International Classification:

G01N033/567

US Classification:

435/007210, 435/270000

Abstract:

A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d.c. impedance volume, conductivity/opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements. Novel methods for use of the reagents with automated and semi-automated hematology analyzers are also provided.

US Patent:

42997267, Nov 10, 1981

Filed:

May 7, 1979

Appl. No.:

6/036794

Inventors:

Harold R. Crews - Miami FL
David L. Chastain - Fort Lauderdale FL
Stephen L. Ledis - Hialeah FL

Assignee:

Coulter Electronics, Inc. - Hialeah FL

International Classification:

G01N 3348
C09K 300

US Classification:

252408

Abstract:

A process for preparing whole blood reference controls having long term stability up to six months for devices using electronic means for whole blood determinations including platelet count and mean cell volume and red blood distribution width. The preconditioning diluent for the red blood cells consists essentially of an aqueous solution of lactose, sodium azide, and a non-ionic surfactant and is pH buffered and osmolality adjusted. The media of the whole blood control includes lactose, fungicides and antibiotics, and supplementary agents including purine nucleosides. It also includes additional components which alter red blood cell membranes including bile salts and cholic acid derivatives, phenothiazine compounds and the salts thereof having antihistamine properties, and 4-aminobenzoic acid ester derivatives and their salts having local anesthetic properties. In the process, the red blood cells are separated from the whole blood and preconditioned by the diluent to attain desired size distribution width and volume. The preconditioned red blood cells then are mixed in the media in suitable number for yielding the desired cell count for the whole blood reference control.

US Patent:

59050314, May 18, 1999

Filed:

Nov 1, 1996

Appl. No.:

8/742620

Inventors:

Nazle Kuylen - Miami FL
Harold R. Crews - Pembroke Pines FL
Gerald E. Jaffe - Pembroke Pines FL
Maria Barcelon - Ft. Lauderdale FL
Francis J. Lucas - Boca Raton FL
Nancy M. Garcia - Miami FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

C12Q1/02

US Classification:

435 29

Abstract:

The necessity to detect blast cells in a patient sample to determine a treatment regime for leukemia has long been desired. The present invention relates to a method and assay reagents for identification of blast cells in a fluid sample. The method further enables the determination of the lineage of the blast cells. The new method provides a determination of blast cells in less time than prior art methods.

US Patent:

55607500, Oct 1, 1996

Filed:

Jun 7, 1995

Appl. No.:

8/488257

Inventors:

Harold R. Crews - Coral Springs FL
Roy M. Evans - Memphis TN
Joseph O. Rubert - Memphis TN

Assignee:

Preemptive Advertising, Inc. - Memphis TN

International Classification:

A61K 713
D06L 300

US Classification:

8431

Abstract:

Disclosed are hair coloring compositions comprising coloring agent and disaccharide. The coloring agent preferably comprises a first oxidizing agent for bleaching the hair, a dyeing agent for adding color to the hair and a second oxidizing agent for developing the dyeing agent. Also disclosed are methods for altering the color of hair to a predetermined degree comprising (a) applying to the hair a hair coloring composition, said composition comprising a coloring agent and a disaccharide; (b) allowing said composition to remain in contact with the hair for a time sufficient to achieve the predetermined degree of color alteration; and (c) substantially removing said composition from the hair.

US Patent:

45215189, Jun 4, 1985

Filed:

Jul 6, 1982

Appl. No.:

6/395530

Inventors:

James H. Carter - Ft. Lauderdale FL
Stephen L. Ledis - Hialeah FL
Harold R. Crews - Miami FL

Assignee:

Coulter Electronics, Inc. - Hialeah FL

International Classification:

G01N 3348
C09K 300

US Classification:

436 10

Abstract:

An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems. This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values. The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.