Harold L Vaughn

Address:

712 Lk Geneva Dr, Saint Augustine, FL 32092

VIN:

4A3AL25F39E032376

Make:

MITSUBISHI

Model:

ECLIPSE SPYDER

Year:

2009

US Patent:

20040171070, Sep 2, 2004

Filed:

Apr 28, 2004

Appl. No.:

10/478910

Inventors:

Ramagauri Bhikhabhai - Uppsala, SE
Maria Liminga - Uppsala, SE
Jean-Luc Maloisel - Uppsala, SE
Ronnie Palmgren - Uppsala, SE
Thomas Keough - Cincinnati OH, US
Amy Keough - Cincinnati OH, US
Robert Youngquist - Mason OH, US
Harold Vaughn - Walton KY, US
Ken Yelm - Fairfield OH, US

International Classification:

G01N033/53

US Classification:

435/007100, 530/410000

Abstract:

The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.