Harry Q Lee

US Patent:

7367550, May 6, 2008

Filed:

Nov 18, 2003

Appl. No.:

10/717409

Inventors:

Harry Lee - Boston MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

B01F 3/04

US Classification:

261 81, 261104, 2611221

Abstract:

The present invention provides devices and methods for achieving mixing and gas exchange in chambers having a small volume. According to the invention a chamber includes one or more walls that are made of a gas-permeable material and that include multiple portions that are selectively deflectable into the interior of the chamber. The deflectable portions are in communication with a hollow cavity or space that can be pressurized. Pressurization results in deflection of the deflectable portion into the chamber. Pressurization is achieved using a gas or fluid that contains a gas of interest such as oxygen. The portions are deflected in a sequence that results in peristaltic action that mixes and oxygenates the contents of the chamber. In a preferred embodiment of the invention the hollow cavities are an assembly of tubes that deflect into a plurality of chambers. A particular use for the invention is as a culture vessel for cells such as bacteria.

US Patent:

7993608, Aug 9, 2011

Filed:

Aug 7, 2008

Appl. No.:

12/188162

Inventors:

Harry Lee - Boston MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

B01L 99/00
G01N 1/38

US Classification:

422546, 73863, 7386463, 422502

Abstract:

Fluid injection port. An elastomeric injection nipple is supported within a compression fitting and the injection nipple includes a slit. A first via is provided that connects the slit in the nipple to a flow channel leading into a fluid reservoir. A venting channel is provided in fluid communication with the fluid reservoir and also in fluid communication with a second via. When a pipette is inserted into the slit in the injection nipple, the nipple deforms allowing the second via to be in fluid communication with space on either side of the pipette tip whereby air can be discharged.

US Patent:

20030053625, Mar 20, 2003

Filed:

Aug 29, 2002

Appl. No.:

10/230652

Inventors:

Theodore Bially - Sudbury MA, US
Harry Lee - Bellevue WA, US

Assignee:

THE TITAN CORPORATION

International Classification:

H04L009/00

US Classification:

380/042000, 380/278000

Abstract:

In an encryption system, a serial data stream is demultiplexed into a plurality N of encryptor input data streams to form a sequence of encryptor input data slices applied to an encryptor having a cascade of stages. Each stage includes a mapping function and a delay function, the mapping function performing a stage-specific direct mapping of data slice values to corresponding generally different data slice values, and the delay function applying stage-specific and generally different delays to individual symbols of data slices. Encrypted data slices generated by the last stage of the encryptor are transmitted through a transmission channel and received at a decryptor having a cascade of stages. Each decryptor stage includes an equalizing delay function and an inverse mapping function to generate output data slices from input data slices. Each output data slice of the last decryptor stage comprises respective values at a given time of a set of N decryptor output data streams, which are multiplexed together to recover the serial data stream.

US Patent:

20080299539, Dec 4, 2008

Filed:

Oct 10, 2006

Appl. No.:

11/545647

Inventors:

Harry Lee - Boston MA, US
Rajeev Ram - Boston MA, US
Klavs Jensen - Lexington MA, US

International Classification:

C12M 3/00
C12M 3/06
C12M 1/36
C12Q 3/00
C12N 1/20
C12Q 1/02

US Classification:

435 3, 4352891, 4352971, 4352865, 4352528, 435 29

Abstract:

In one embodiment, the present invention introduces integrated fluid injection and mixing devices to enable pH control in a miniature parallel integrated bioreactor array system. In another embodiment, the environmental conditions of the growth chamber is enabled through fluidic injections in a miniature parallel cell culture system. In still another embodiment, the present invention utilizes gas switches to control oxygen concentration within a miniature parallel integrated bioreactor array.

US Patent:

20090220935, Sep 3, 2009

Filed:

Mar 9, 2007

Appl. No.:

12/281919

Inventors:

Harry Lee - Boston MA, US
Kevin Shao-Kwan Lee - Cambridge MA, US

Assignee:

Massachusetts Instutite of Technology - Cambridge MA

International Classification:

C12Q 3/00
C12M 1/00
C40B 40/00

US Classification:

435 3, 4352971, 506 13

Abstract:

Parallel, integrated bioreactor. The bioreactor includes a plurality of growth chambers, each growth chamber associated with a peristaltic oxygenating mixer and separated therefrom by a porous membrane. Each oxygenating mixture has a gas inlet and a gas outlet with the gas inlet in fluid communication with a gas reservoir. A gas mixer switch is provided for controlling oxygen concentration in the reservoir. The apparatus and methods disclosed in the application allow precise control over dissolved oxygen concentration with a quick response time.

US Patent:

20110137381, Jun 9, 2011

Filed:

Jan 2, 2009

Appl. No.:

12/319158

Inventors:

Harry Lee - Boston MA, US
Allan Foreman - Epping NH, US
Wolfgang Daum - Groton MA, US
Richard Cohen - Chestnut Hill MA, US
Jean Gotman - Westmount, CA
Andrew James Cole - Lincoln MA, US

International Classification:

A61N 1/37

US Classification:

607 62

Abstract:

Disclosed are medical devices for the prevention and/or treatment of neurological disorders via electrical stimulation, and methods related thereto. The devices may also be utilized to detect disorders before the prevention or treatment of a neurological disorder. These devices are minimally or non-invasive. The present medical devices comprise various components, which include electrodes and control electronics. The electrodes are targeting electrodes constructed from ring type structures or virtually connected disc type arrays. The electrodes are located entirely outside the skull. The present medical devices also comprise one or more subsystems, a control system, a battery unit, and wires connecting the one or more subsystems. These devices may also be used for acute seizure control.

US Patent:

20110195260, Aug 11, 2011

Filed:

Oct 9, 2009

Appl. No.:

13/123310

Inventors:

S. Kevin Lee - Cambridge MA, US
Harry Lee - Boston MA, US
J. Rajeev Ram - Cambridge MA, US

International Classification:

B32B 9/04
B32B 37/02
B32B 37/06
B32B 37/12
B32B 37/16
B01L 3/00
G01N 33/48
C07F 7/08
C09D 7/00
B82Y 99/00

US Classification:

428447, 156325, 1562726, 156329, 422502, 422 681, 556449, 10628713, 977700

Abstract:

Active devices such as pumps and mixers have been fabricated in plastic-PDMS hybrid devices. By utilizing functionalized bis-silane primers, bond strength between Polycarbonate or PMMA and PDMS improved in dry and aqueous environments. Plastic-primer-PDMS layers exposed to acid and base solutions at 70 C. for 2 hours showed no signs of delamination at 30 psi for pH −1 to 15 and 60 psi for pH 0 to 15. A peristaltic pump fabricated in polycarbonate achieved consistent flow rates up to peristaltic cycle frequencies of 10 Hz in water, 1OM HCl, and 1OM NaOH solutions.

US Patent:

20130194575, Aug 1, 2013

Filed:

Apr 9, 2012

Appl. No.:

13/442783

Inventors:

Harry Lee - Boston MA, US

International Classification:

G01N 21/59

US Classification:

356436

Abstract:

The present invention provides apparatus and methods for measuring the optical density of a fluid by measuring the light transmission through a reconfigurable optical path. The optical path is reconfigured by the deflection of an elastic membrane wherein the optical path length through the fluid is determined by the position of the membrane relative to a fixed surface. The optical density can be determined from two measurements with different optical path lengths through the fluid.