Heather J Grant

US Patent:

20210003559, Jan 7, 2021

Filed:

Jun 25, 2020

Appl. No.:

16/912391

Inventors:

- Little Falls NJ, US
- Basel, CH
- Boston MA, US
Annie Moisan - Basel, CH
Nikolche Gjorevski - Basel, CH
Jordan S. Kerns - Boston MA, US
Geraldine A. Hamilton - Boston MA, US
Catherine Karalis - Boston MA, US
Heather Grant - Boston MA, US
Riccardo Barrile - Boston MA, US
Debora Barreiros Petropolis - Boston MA, US
Chaitra Belgur - Boston MA, US

International Classification:

G01N 33/50

Abstract:

Disclosed herein is a method for assessing a compound interacting with a target on polarized epithelial cells. The method comprising the steps of providing an organ chip comprising a main channel and polarized epithelial cells, wherein the main channel is divided into an apical channel and a basal channel separated by the polarized epithelial cells, wherein the apical side of the polarized epithelial cells is directed towards the apical channel and the basolateral side of the polarized epithelial cells is directed towards the basal channel. Determining the localization and optionally the expression level of the target on the polarized epithelial cells. Administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the basal channel, when the target is localized on the basolateral side of the epithelial cells or administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the apical channel, when the target is localized on the apical side of the epithelial cells. Measuring a parameter of the administration of the compound and the peripheral blood mononuclear cells.