Heather E O'Keefe

US Patent:

7060432, Jun 13, 2006

Filed:

Jun 14, 2000

Appl. No.:

09/593914

Inventors:

Heather P. O'Keefe - Lexington MA, US
Henrik Stender - Gentofte, DK

Assignee:

Applera Corporation - Framingham MA

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 435 405, 435 4051, 536 231, 536 2374, 536 243, 536 2432

Abstract:

This invention is related to novel probes, probe sets, methods and kits pertaining to the detection, identification and/or quantitation of yeasts and particularly (a. k. a. ) an organism that spoils wine. Preferred probes for the detection of one or more species of the genus comprise a probing nucleobase sequence, at least a portion of which is selected from the group consisting of: AGC-GGG-TCT-ATT-AGA (Seq. ID No. 1); CCA-GGT-GAG-GGT-CGC (Seq. ID No. 2); CGG-TTG-CCC-GAT-TTC (Seq. ID No. 3); TCG-CCT-TCC-TCC-TCT (Seq. ID No. 4); CGG-TCT-CCA-GCG-ATT (Seq. ID No. 5); CAC-AAG-ATG-TCC-GCG (Seq. ID No. 6); GCG-GGC-ACT-AAT-TGA (Seq. ID No. 7); CAT-CCA-CGA-GGA-ACG (Seq. ID No. 8); GTG-TAA-ACC-AGG-TGC (Seq. ID No. 9); ATG-GCT-CCC-AGA-ACC (Seq. ID No. 10) and GAC-AGA-ATC-GAA-GGG (Seq. ID No. 11).

US Patent:

7108980, Sep 19, 2006

Filed:

Oct 14, 2003

Appl. No.:

10/684971

Inventors:

Heather P. O'Keefe - Lexington MA, US

Assignee:

Boston Probes, Inc. - Bedford MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

This invention is related to methods pertaining to the determination of bacteria and yeast wherein the methods comprise: a) filtering a sample containing bacteria and yeast through at least one filter; b) treating the filter or filters with a medium which facilitates the growth of the bacteria and yeast; c) growing the bacteria and yeast on the filter or filters to thereby produce colonies or microcolonies of the bacteria and yeast; d) fixing the colonies or microcolonies to the filter or filters; e) treating each filter under suitable hybridization conditions with one or more enzyme labeled PNA probes wherein, each PNA probe comprises a probing nucleobase sequence complementary to a target sequence in the nucleic acid of the bacteria and/or yeast; f) removing excess enzyme labeled PNA probe from each of the filters; and g) determining enzyme activity remaining on each filter to thereby identify or enumerate colonies or microcolonies on the filter or filters.

US Patent:

62809466, Aug 28, 2001

Filed:

Aug 3, 1999

Appl. No.:

9/368089

Inventors:

Heather P. O'Keefe - Lexington MA

Assignee:

Boston Probes, Inc. - Bedford MA

International Classification:

C12Q 168
C07H 2104

US Classification:

435 6

Abstract:

This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and/or eucarya. Preferred universal probes for the detection of bacteria comprise a probing nucleobase sequence selected from the group consisting of CTG-CCT-CCC-GTA-GGA; TAC-CAG-GGT-ATC-TAA-T; CAC-GAG-CTG-ACG-ACA and CCG-ACA-AGG-AAT-TTC. Preferred universal probes for the detection of eucarya comprise a probing nucleobase sequence selected from the group consisting of ACC-AGA-CTT-GCC-CTC-C; GGG-CAT-CAC-AGA-CCT-G; TAG-AAA-GGG-CAG-GGA and TAC-AAA-GGG-CAG-GGA. The PNA probes, probe sets, methods and kits of this invention are particularly well suited for use in multiplex PNA-FISH assays wherein the bacteria and/or eucarya in a sample can be individually detected, identified or quantitated. Using exemplary assays described herein, the total number of colony forming units (CFU) of bacteria and/or eucarya can be rapidly determined.