Heather J O'Keefe from Ware, MA, age 44

Methods And Kit For Hybridization Analysis Using Peptide Nucleic Acid Probes
view source
US Patent:

20020012902, Jan 31, 2002
Filed:

Oct 4, 1996
Appl. No.:

08/726093
Inventors:

MARTIN FUCHS - UXBRIDGE MA, US
MICHAEL EGHOLM - LEXINGTON MA, US
HEATHER O'KEEFE - LEXINGTON MA, US
International Classification:

C12Q001/00
G01N031/00
G01N033/53
C25D021/12
US Classification:

435/004000, 436/516000, 435/007100, 205/081000
Abstract:

A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

Methods And Kit For Hybridization Anaylsis Using Peptide Nucleic Acid Probes
view source
US Patent:

20050053944, Mar 10, 2005
Filed:

Aug 7, 2003
Appl. No.:

10/636343
Inventors:

Martin Fuchs - Uxbridge MA, US
Michael Egholm - Woodbridge CT, US
Heather O'Keefe - Lexington MA, US
International Classification:

C12Q001/68
C07K014/47
US Classification:

435006000, 530350000
Abstract:

A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.