Heather Allene Shepherd

US Patent:

20110206578, Aug 25, 2011

Filed:

Feb 23, 2011

Appl. No.:

13/033206

Inventors:

Evan Foster - San Mateo CA, US
Jessica Reed - Newark CA, US
Heather Shepherd - Oakland CA, US
Christina Inman - San Mateo CA, US
Scott Benson - Alameda CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

B01L 9/00
B05D 3/10
B05D 3/06
B05D 7/22
B05D 3/00

US Classification:

422563, 427331, 427343, 427539, 427299, 427230, 427444

Abstract:

Methods for activating and/or reactivating a metal oxide surface for deposition, immobilizing, and/or growing a biological sample (e.g., an oligonucleotide, a polynucleotide, functionalized particle, a polymer, etc.) thereon are disclosed. The metal oxide surface (e.g., zirconium oxide) can be activated and/or reactivated by exposing the metal oxide surface to various activation and/or treatment protocols capable of enhancing a slide's activity relative to the activity of an untreated slide. The activation and/or treatment protocol can include subjecting the slide (or at least the metal oxide layer) to an oxygen plasma treatment and/or a peroxide solution for an amount of time sufficient to activate and/or reactivate the metal oxide surface. Activated sequencing slides capable of incorporation, for example, into various embodiments of a flow cell for use in NGS platforms are also disclosed herein.