Hehuang B Xie from Blacksburg, VA, age 43

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Location:

Blacksburg, VA

Industry:

Higher Education

Work:

Virginia Tech
Associate Professor

Skills:

Genetics
Molecular Biology
Research
Cell Culture
Cell Biology
Life Sciences
Biochemistry
Higher Education
Pcr
Teaching

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Selective Enrichment Of Cpg Islands
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US Patent:

8278049, Oct 2, 2012
Filed:

Apr 26, 2010
Appl. No.:

12/767382
Inventors:

Hehuang Xie - Chicago IL, US
Assignee:

Ann & Robert H. Lurie Children's Hospital of Chicago - Chicago IL
International Classification:

C12Q 1/68
US Classification:

435 611, 435 61
Abstract:

The present invention provides compositions and methods for selectively enriching genomic CpG island (CGI)- and other epigenetically informative CG-rich polynucleotide targets. The method involves co-incubation of denatured or partially denatured polynucleotide fragments containing the CGI- or CG-targeted region(s) of interest with an oligonucleotide capture pool collectively designed to selectively target CGIs. The oligonucleotide capture pool includes a plurality of different oligonucleotides, each oligonucleotide coupled to a capture tag, whereby the oligonucleotide includes a CpG target sequence restricted to 4 to 10 bases. Upon binding, capture oligonucleotides bound to the target fragments are enriched by separating the bound fragments from the unbound fragments. The enriched fragments may be subjected to further analyses, including bisulfite sequencing to generate a methylation profile at the single nucleotide level.

Selective Enrichment Of Cpg Islands
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US Patent:

20130090262, Apr 11, 2013
Filed:

Oct 2, 2012
Appl. No.:

13/633285
Inventors:

Ann and Robert H. Lurie Children's Hospital of Chi - Chicago IL, US
Hehuang Xie - Blacksburg VA, US
Assignee:

Ann and Robert H. Lurie Children's Hospital of Chicago - Chicago IL
International Classification:

C12Q 1/68
US Classification:

506 16
Abstract:

The present invention provides compositions and methods for selectively enriching genomic CpG island (CGI)- and other epigenetically informative CG-rich polynucleotide targets. The method involves co-incubation of denatured or partially denatured polynucleotide fragments containing the CGI- or CG-targeted region(s) of interest with an oligonucleotide capture pool collectively designed to selectively target CGIs. The oligonucleotide capture pool includes a plurality of different oligonucleotides, each oligonucleotide coupled to a capture tag, whereby the oligonucleotide includes a CpG target sequence restricted to 4 to 10 bases. Upon binding, capture oligonucleotides bound to the target fragments are enriched by separating the bound fragments from the unbound fragments. The enriched fragments may be subjected to further analyses, including bisulfite sequencing to generate a methylation profile at the single nucleotide level.

Hehuang B Xie