Hui Zhang

US Patent:

7198896, Apr 3, 2007

Filed:

Jun 19, 2002

Appl. No.:

10/175486

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Beverly MA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

Assignee:

Cell Signaling Technology, Inc. - Danvers MA

International Classification:

C12Q 1/00
C12Q 1/37
C12P 21/06
G01N 33/543

US Classification:

435 6, 435 4, 435 691, 435 24, 435174, 436518, 436 89, 436501, 216601, 216661, 216656

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

US Patent:

7300753, Nov 27, 2007

Filed:

Feb 12, 2004

Appl. No.:

10/777893

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Iowa City IA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

International Classification:

C12Q 1/00
C12Q 1/37
C12P 21/06
G01N 33/543

US Classification:

435 6, 435 4, 435 691, 435 24, 435174, 436518, 436 89, 436501, 216601, 216661, 216656

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

US Patent:

7426575, Sep 16, 2008

Filed:

May 14, 2001

Appl. No.:

09/859192

Inventors:

Bob Reissfelder - Westwood MA, US
Yantao Jia - Stow MA, US
Lei Jin - Irvine CA, US
Shahzad Ali - Rohnert Park CA, US
Stephen John West - Petaluma CA, US
Hui Zhang - Santa Rosa CA, US
Shuangxia Sarah Zhu - Rohnert Park CA, US

Assignee:

Turin Networks - Petaluma CA

International Classification:

G06F 15/173
G06F 15/16

US Classification:

709240, 709231, 709238

Abstract:

An arriving cell is admitted when a flow occupancy level is less than a flow minimum threshold. When the flow occupancy level is not less than the flow minimum threshold, a drop probability based on per-class thresholds is applied to determine if the arriving is to be rejected. When the arriving cell is not to be rejected after applying the drop probability based on the per-class thresholds, a drop probability based on per-flow thresholds is applied.

US Patent:

20110053242, Mar 3, 2011

Filed:

Jun 28, 2007

Appl. No.:

11/823775

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Iowa City IA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

International Classification:

C12N 11/00
C12S 3/14
G01N 33/566
C07K 1/14
C07K 16/18
C07K 16/00
C07K 16/40
C07K 1/22
H01J 49/26
G01N 30/72
B01D 11/02

US Classification:

435174, 435272, 436501, 530344, 5303898, 5303911, 250282, 73 6152, 422261

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.