Hui H Zhang

US Patent:

7198896, Apr 3, 2007

Filed:

Jun 19, 2002

Appl. No.:

10/175486

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Beverly MA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

Assignee:

Cell Signaling Technology, Inc. - Danvers MA

International Classification:

C12Q 1/00
C12Q 1/37
C12P 21/06
G01N 33/543

US Classification:

435 6, 435 4, 435 691, 435 24, 435174, 436518, 436 89, 436501, 216601, 216661, 216656

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

US Patent:

7259022, Aug 21, 2007

Filed:

Nov 13, 2001

Appl. No.:

10/014485

Inventors:

Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US
Hui Zhang - Seattle WA, US

Assignee:

Cell Signaling Technology, Inc. - Danvers MA

International Classification:

A61K 38/00
C07K 16/00

US Classification:

436547, 436548, 5303871, 5303881

Abstract:

A method is provided for producing motif-specific, context-independent antibodies which recognize a plurality of peptides or proteins within a genome that contain the same motif. The method includes the step of immunizing a host with a degenerate peptide library antigen featuring (i) a fixed target motif containing one or more invariant amino acids including at least one modified amino acid, and (ii) a plurality of degenerate amino adds flanking the motif. Motif-specific, context-independent antibodies produced by the disclosed method are also provided. The method encompasses motifs consisting of a single modified amino acid, as well as short motifs comprising multiple invariant amino acids including one or more modified amino acids, such as all or part of kinase consensus substrate motifs, protein-protein binding motifs, or other cell signaling motifs. Methods of using the antibodies, e. g. for genome-wide profiling, are also provided.

US Patent:

7300753, Nov 27, 2007

Filed:

Feb 12, 2004

Appl. No.:

10/777893

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Iowa City IA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

International Classification:

C12Q 1/00
C12Q 1/37
C12P 21/06
G01N 33/543

US Classification:

435 6, 435 4, 435 691, 435 24, 435174, 436518, 436 89, 436501, 216601, 216661, 216656

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

US Patent:

7426575, Sep 16, 2008

Filed:

May 14, 2001

Appl. No.:

09/859192

Inventors:

Bob Reissfelder - Westwood MA, US
Yantao Jia - Stow MA, US
Lei Jin - Irvine CA, US
Shahzad Ali - Rohnert Park CA, US
Stephen John West - Petaluma CA, US
Hui Zhang - Santa Rosa CA, US
Shuangxia Sarah Zhu - Rohnert Park CA, US

Assignee:

Turin Networks - Petaluma CA

International Classification:

G06F 15/173
G06F 15/16

US Classification:

709240, 709231, 709238

Abstract:

An arriving cell is admitted when a flow occupancy level is less than a flow minimum threshold. When the flow occupancy level is not less than the flow minimum threshold, a drop probability based on per-class thresholds is applied to determine if the arriving is to be rejected. When the arriving cell is not to be rejected after applying the drop probability based on the per-class thresholds, a drop probability based on per-flow thresholds is applied.

US Patent:

20030068652, Apr 10, 2003

Filed:

Jun 18, 2002

Appl. No.:

10/174105

Inventors:

Michael Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US
Hui Zhang - Seattle WA, US

Assignee:

Cell Signaling Technology, Inc.

International Classification:

G01N033/53
G06F019/00
G01N033/48
G01N033/50

US Classification:

435/007100, 702/019000, 436/518000, 204/461000

Abstract:

The invention provides a method for producing antibodies that selectively recognize short, modified amino acid motifs substantially independent of the surrounding amino acid context in which the motif occurs. A novel class of motif-specific, context-independent antibodies is also provided. The invention encompasses modified motifs consisting of single modified amino acids, for example phosphotyrosine or acetylated lysine, as well other modified motifs of multiple amino acids, such as kinase consensus substrate motifs and protein-protein binding motifs relevant to cell signal transduction. Also provided are methods of profiling large and diverse protein populations on a genome-wide basis by utilizing the antibodies of the invention, and methods for the positive identification of cellular phosphoproteins using one or more motif-specific, context-independent antibodies of the invention coupled with protein database searching.

US Patent:

20110053242, Mar 3, 2011

Filed:

Jun 28, 2007

Appl. No.:

11/823775

Inventors:

John Rush - Brookline MA, US
Hui Zhang - Seattle WA, US
Xiangming Zha - Iowa City IA, US
Michael J. Comb - Manchester MA, US
Yi Tan - Lynnfield MA, US

International Classification:

C12N 11/00
C12S 3/14
G01N 33/566
C07K 1/14
C07K 16/18
C07K 16/00
C07K 16/40
C07K 1/22
H01J 49/26
G01N 30/72
B01D 11/02

US Classification:

435174, 435272, 436501, 530344, 5303898, 5303911, 250282, 73 6152, 422261

Abstract:

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

US Patent:

20200255969, Aug 13, 2020

Filed:

Apr 27, 2020

Appl. No.:

16/859168

Inventors:

- Hudson NH, US
Cody Riopel - North Reading MA, US
Hui Zhang - Nashua NH, US

International Classification:

C30B 11/00
C30B 29/20
C30B 29/06

Abstract:

A method of producing a crystalline material is provided that may include providing a crystal growth apparatus comprising a chamber, a hot zone, and a muffle. The hot zone may be disposed within the chamber and include at least one heating system, at least one heat removal system, and a crucible containing feedstock. Additionally, the method may include providing a muffle that surrounds at least two sides of the crucible to ensure uniform temperature distribution through the feedstock during crystal growth to allow the crystalline material to be grown with a square or rectangular shaped cross section.

US Patent:

20180057957, Mar 1, 2018

Filed:

Oct 30, 2017

Appl. No.:

15/797833

Inventors:

- Merrimack NH, US
Hui Zhang - Nashua NH, US
Ning Duanmu - Nashua NH, US
Ian T. Witting - Goffstown NH, US

International Classification:

C30B 11/00
C30B 35/00
C30B 29/20
C30B 15/14

Abstract:

An advanced crucible support system is described that allows for greater heat flow to and from the bottom of a crucible, preferably while also preventing excessive heat from reaching a heat exchanger. In particular, a support base is described that includes a plurality of spaced crown features disposed on the support base plate. The crown features receive and vertically support the crucible and are spaced to support the crucible and to allow heat flow between the plurality of crown features. In doing so, a top surface of spaced crown features are in direct contact with the crucible.