Jacqueline N Ward

US Patent:

20040038373, Feb 26, 2004

Filed:

Dec 9, 2002

Appl. No.:

10/314869

Inventors:

Matthew Platz - Columbus OH, US
Michael Caligiuri - Upper Arlington OH, US
Susan Olesik - Dublin OH, US
Helene Balladur - Columbus OH, US
Jacqueline Ward - Columbus OH, US

International Classification:

C12N015/86
C12N007/00

US Classification:

435/235100, 435/239000, 435/456000

Abstract:

Cell preparations comprising a plurality of apoptotic EBV-transformed B lymphocytes, and methods of producing cell preparations comprising a plurality of apoptotic EBV-transformed B lymphocytes are provided. The methods comprise transforming B lymphocytes with EBV, incubating the transformed B lymphocytes with a flavin photosensitizer, such as riboflavin or a lumichrome-resistant photosenstizer, adding a non-toxic anti-oxidant, and exposing the lymphocytes to photoradiation of an appropriate wavelength to activate the photosensitizer. Also provided are methods of using the apoptotic EBV-transformed B lymphocyte cell preparations to elicit production of EBV-specific T cells in human patients. Finally, methods of treating organ transplant patients comprising administering an effective amount of the apoptotic EBV-transformed B-lymphocytes cell preparation to the patients prior to transplantation are provided.