James Lawrence Burg

US Patent:

6410276, Jun 25, 2002

Filed:

Apr 24, 1995

Appl. No.:

08/427606

Inventors:

James Lawrence Burg - Foster City CA
Philippe Jacques Pouletty - Menlo Park CA
John Charles Boothroyd - Palo Alto CA

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

C12P 1934

US Classification:

435 912, 435 6, 435 911, 536 243

Abstract:

A method is provided for multiplying the number of copies of a target polynucleotide sequence comprising a series of primer hybridization, extending, and denaturing steps to provide an intermediate double-stranded DNA molecule containing a promoter sequence (through the use of a promoter-sequence-containing primer) incorporated upstream from the target sequence. The double-stranded DNA intermediate is then used to grow multiple RNA copies of the target sequence. The resulting RNA copies can be used as target sequences to produce further copies. Multiple cycles of this sort can thereby exponentially increase the number of target sequence copies.

US Patent:

20130157378, Jun 20, 2013

Filed:

Dec 15, 2011

Appl. No.:

13/326937

Inventors:

James Lawrence Burg - Framingham MA, US

International Classification:

G01N 21/64

US Classification:

436501, 422 69

Abstract:

A device including a shallow chamber for analyzing a plurality of target analytes in a body fluid using the signal generated by fluorescent detector molecules each specific for a target analyte, an attenuating dye for attenuating the signal emitted by fluorescent detector molecules specifically bound to the surfaces of the chamber other than an optically clear surface, and method for determining the signal generated by each of the plurality of analytes.

US Patent:

58591966, Jan 12, 1999

Filed:

Jun 5, 1995

Appl. No.:

8/461296

Inventors:

John Charles Boothroyd - Palo Alto CA
James Lawrence Burg - Foster City CA
Lloyd H. Kasper - Norwich VT

Assignee:

Trustees of the Leland Stanford Junior University - Hanover NH
Trustees of Dartmouth College - Stanford CA

International Classification:

C12N 1563
C12N 510

US Classification:

530350

Abstract:

Genetic material encoding p30 and B1 peptides of Toxoplasma gondii has been isolated and characterized. This genetic material allows the production of peptides for use in diagnosis or immunization or can itself be directly used in hybridization assays.

US Patent:

56294140, May 13, 1997

Filed:

Jan 14, 1994

Appl. No.:

8/182673

Inventors:

John C. Boothroyd - Palo Alto CA
James L. Burg - Foster City CA
Lloyd H. Kasper - Norwich VT

Assignee:

The Board of Trustees of the Leeland Stanford Junior University - Stanford CA

International Classification:

C07H 2100
C12N 120
C12N 1500

US Classification:

536 237

Abstract:

Genetic material encoding p30 and B1 peptides of Toxoplasma gondii has been isolated and characterized. This genetic material allows the production of peptides for use in diagnosis or immunization or can itself be directly used in hybridization assays.

US Patent:

54379903, Aug 1, 1995

Filed:

Sep 13, 1993

Appl. No.:

8/121034

Inventors:

James L. Burg - Foster City CA
Philippe J. Pouletty - Menlo Park CA
John C. Boothroyd - Palo Alto CA

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

C12P 1934
C07H 2102
C07H 2104

US Classification:

435 912

Abstract:

A method is provided for multiplying the number of copies of a target polynucleotide sequence comprising a series of primer hybridization, extending, and denaturing steps to provide an intermediate double-stranded DNA molecule containing a promoter sequence (through the use of a promoter-sequence-containing primer) incorporated upstream from the target sequence. The double-stranded DNA intermediate is then used to grow multiple RNA copies of the target sequence. The resulting RNA copies can be used as target sequences to produce further copies. Multiple cycles of this sort can thereby exponentially increase the number of target sequence copies.