James D Higginbotham

US Patent:

6753142, Jun 22, 2004

Filed:

Mar 19, 2001

Appl. No.:

09/811842

Inventors:

Charles J. Link - Des Moines IA
Tatiana Seregina - Ames IA
Nicholas N. Vahanian - Ames IA
James N. Higginbotham - Ames IA
W. Jay Ramsey - Ames IA
Bradley J. Powers - Ames IA
Sachet A. Shukla - Ames IA
Won Bin Young - Ames IA

Assignee:

NewLink Genetics Inc. - Ames IA

International Classification:

C12Q 168

US Classification:

435 6, 435 691, 435463, 4353201

Abstract:

The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

US Patent:

6875610, Apr 5, 2005

Filed:

May 31, 2001

Appl. No.:

09/871183

Inventors:

James N. Higginbotham - Ames IA, US
Charles J. Link - Des Moines IA, US
William J. Ramsey - Ames IA, US

Assignee:

Human Gene Therapy Research Institute - Des Moines IA

International Classification:

C12N015/861
C12N015/63
C12N015/64
C12N005/10
C07H021/04

US Classification:

435457, 4353201, 435455, 435456, 435325, 435 691, 536 231, 536 235, 536 232, 536 237, 536 241

Abstract:

The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

US Patent:

6897020, May 24, 2005

Filed:

Mar 19, 2001

Appl. No.:

09/811842

Inventors:

Charles J. Link - Des Moines IA, US
Tatiana Seregina - Ames IA, US
Nicholas N. Vahanian - Ames IA, US
James N. Higginbotham - Ames IA, US
W. Jay Ramsey - Ames IA, US
Bradley J. Powers - Ames IA, US
Sachet A. Shukla - Ames IA, US
Won Bin Young - Ames IA, US

Assignee:

NewLink Genetics Inc. - Ames IA

International Classification:

C12Q001/68

US Classification:

435 6, 435 691, 435463, 4353201

Abstract:

The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

US Patent:

7078179, Jul 18, 2006

Filed:

May 4, 2004

Appl. No.:

10/838750

Inventors:

Teresa Di Colandrea - Ames IA, US
Cherisa Meyer - Ames IA, US
Won-Bin Young - Ames IA, US
James N. Higginbotham - Hendersonville TN, US
Mario Mautino - Ames IA, US

Assignee:

Newlink Genetics Corporation - Ames IA

International Classification:

G01N 33/567
G01N 33/53
C12Q 1/68
C12N 15/63
C12N 15/86

US Classification:

435 721, 435 6, 435 71, 435455, 435456

Abstract:

A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-α Galactosyltransferase (αGT) enzyme for the production of αGalactosyl-(1,3)Galactosyl (αGal) epitopes on the surface of αGal-negative cells. These cells which are genetically modified to express the αGT enzyme and αGal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the αGal epitopes on the cell surface. Cells labeled with the anti-αGal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of αGal-positive cells from within a population of αGal-negative cells without the need to expose cells to antibiotics for any period of time. In addition, the specification provides a method for the production and purification of anti-αGal antibodies from chicken egg yolk.

US Patent:

7125549, Oct 24, 2006

Filed:

May 31, 2001

Appl. No.:

10/297099

Inventors:

James N. Higginbotham - Ames IA, US
William J. Ramsey - Ames IA, US

Assignee:

Human Gene Therapy Research Institute - Des Moines IA

International Classification:

C12N 15/63
C12N 15/64
C12N 15/861
C12N 15/867
C12N 15/869

US Classification:

424 932, 424 931, 424 936, 4353201, 435455456, 435457

Abstract:

The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

US Patent:

20060134629, Jun 22, 2006

Filed:

Sep 12, 2003

Appl. No.:

10/660893

Inventors:

Charles Link - West Des Moines IA, US
Tatiana Seregina - West Des Moines IA, US
Nicholas Vahanian - Ankeny IA, US
James Higginbotham - Nashville TN, US
William Ramsey - Ankeny IA, US
Bradley Powers - Ames IA, US
Sachet Shukla - Ames IA, US
Won Young - Des Moines IA, US
Teresa Dicolandrea - Ames IA, US
Mario Mautino - Ankeny IA, US

International Classification:

C12Q 1/68
G01N 33/567
G01N 33/53

US Classification:

435006000, 435007200

Abstract:

The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.