James W Kwan

US Patent:

20110300078, Dec 8, 2011

Filed:

Mar 9, 2011

Appl. No.:

13/044224

Inventors:

Mark A. Borden - New York NY, US
Jameel Adebayo Feshitan - New York NY, US
Cherry Chen - New York NY, US
Shashank Ramesh Sirsi - New York NY, US
James Jing Kwan - Hartsdale NY, US

Assignee:

THE TRUSTEES OF CLUMBIA UNIVERSITY IN THE CITY OF NEW YORK - Broadway NY

International Classification:

A61K 49/18
A61K 9/00
B01D 43/00

US Classification:

424 952, 494 37, 424400

Abstract:

In one aspect of the disclosed subject matter, a method for isolating target microbubbles having a predetermined size range from polydisperse microbubbles is disclosed. The method includes applying a first centrifugal field having a first field strength to a suspension comprising the polydisperse microbubbles for a first duration of time, thereby forming a first infranatant comprising at least a portion of target microbubbles and a first supernatant cake comprising microbubbles having a greater size than the target microbubbles; removing the first supernatant cake; applying a second centrifugal field having a second field strength to the first infranatant for a second duration of time, the second field strength being greater than the first field strength, thereby forming a second supernatant cake comprising at least a portion of the target microbubbles and second infranatant comprising microbubbles having a smaller size than the target microbubbles; and isolating the second supernatant cake. In another aspect of the disclosed subject matter, a method for performing high frequency ultrasonic imaging using isolated microbubbles is provided.