James A Lautenberger

US Patent:

52368289, Aug 17, 1993

Filed:

Jan 30, 1992

Appl. No.:

7/827877

Inventors:

Takis S. Papas - Potomac MD
James A. Lautenberger - Rockville MD

International Classification:

C12N 1573
C12N 1563
C12N 1566

US Classification:

435 691

Abstract:

The plasmid of this invention contains the cII translation initiation site and a P. sub. L promoter--both are very efficient, producing large amounts of cII protein. This plasmid contains a unique ClaI restriction site in the aminoterminal portion of the cII gene suitable for insertion of foreign genes. This plasmid is an improvement over other plasmids because the inserted genes are subject to the same transcriptional and translational characteristics of the cII gene; this plasmid, therefore, produces large amounts of the protein coded by the exogenous gene.

US Patent:

56747055, Oct 7, 1997

Filed:

Feb 14, 1994

Appl. No.:

8/194818

Inventors:

Takis S. Papas - Potomac MD
Kenneth Samuel - Hyattsville MD
James A. Lautenberger - Middletown MD
Flossie Wong-Staal - Pontomac MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12P 2106
C12Q 170
G01N 3353
C07K 100

US Classification:

435 691

Abstract:

Antigenic proteins may be expressed in bacteria by use of vectors having inserted therein DNA fragments from an envelope gene. The DNA fragments employed in the example are coding sequences found in the HTLV-I envelope gene. The bacteria used was E. coli. The antigenic proteins are useful in identifying antibodies to the organisms from which the DNA fragments were originally obtained.

US Patent:

55102387, Apr 23, 1996

Filed:

Feb 7, 1994

Appl. No.:

8/193510

Inventors:

Takis S. Papas - Potomac MD
Kenneth Samuel - Hyattsville MD
James A. Lautenberger - Middletown MD
Flossie Wong-Staal - Potomac MD

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12P 2106
C12Q 170
C07K 100
G01N 3353

US Classification:

435 5

Abstract:

Antigenic proteins may be expressed in bacteria by use of vectors having inserted therein DNA fragments from an envelope gene. The DNA fragments employed in the example are coding sequences found in the HTLV-I envelope gene. The bacteria used was E. coli. The antigenic proteins are useful in identifying antibodies to the organisms from which the DNA fragments were originally obtained.