James C Mercer

Address:

159 W Lndg, Williamsburg, VA 23185

Phone:

7572530065

VIN:

1GNFK13047R194499

Make:

CHEVROLET

Model:

TAHOE

Year:

2007

US Patent:

7294484, Nov 13, 2007

Filed:

Aug 25, 2005

Appl. No.:

11/213308

Inventors:

Denis Drapeau - Salem NH, US
Yen-Tung Luan - Chelmsford MA, US
James R. Mercer - Derry NH, US
Wenge Wang - North Chelmsford MA, US
Daniel R. Lasko - Medford MA, US

Assignee:

Wyeth Research Ireland Limited - Little Connell, Newbridge, County Kildare

International Classification:

C12P 21/06

US Classification:

435 691

Abstract:

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0. 2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0. 4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

US Patent:

7300773, Nov 27, 2007

Filed:

Aug 25, 2005

Appl. No.:

11/213633

Inventors:

Denis Drapeau - Salem NH, US
Yen-Tung Luan - Chelmsford MA, US
James R. Mercer - Derry NH, US
Wenge Wang - North Chelmsford MA, US
Daniel R. Lasko - Medford MA, US

Assignee:

Wyeth Research Ireland Limited - Little Connell, Newbridge, County Kildare

International Classification:

C12P 21/06

US Classification:

435 691

Abstract:

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0. 2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0. 4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

US Patent:

7335491, Feb 26, 2008

Filed:

Aug 25, 2005

Appl. No.:

11/213317

Inventors:

Denis Drapeau - Salem NH, US
Yen-Tung Luan - Chelmsford MA, US
James R. Mercer - Derry NH, US
Wenge Wang - North Chelmsford MA, US
Daniel R. Lasko - Medford MA, US

Assignee:

Wyeth Research Ireland Limited - Little Connell, Newbridge, County Kildere

International Classification:

C12P 21/06

US Classification:

435 691, 435 701, 435325, 530350

Abstract:

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0. 2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0. 4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

US Patent:

20030005808, Jan 9, 2003

Filed:

Jul 9, 2001

Appl. No.:

09/901537

Inventors:

James Mercer - Hampton VA, US

International Classification:

G10D003/04

US Classification:

084/307000

Abstract:

The present invention transfers sound vibrations to all parts of the violin, or henceforth any member of the violin family, in a more efficient way. As to transmit the sound or vibrations with less loss of these sound vibrations. This system gives members of the violin family a clearer, softer, yet louder more beautiful tone. It consists of three parts: a new and better bridge, a different and better sound post, and a newly designed tailpiece.

US Patent:

20040129127, Jul 8, 2004

Filed:

Aug 18, 2003

Appl. No.:

10/642853

Inventors:

James Mercer - Newport News VA, US

International Classification:

G10D003/04

US Classification:

084/307000

Abstract:

An apparatus adding one new component and replacing four outdated parts with enhanced components, which have characteristics that efficiently transfer and recycle sound vibrations, have correct wood grain direction for optimal sound flow, and are designed with “lower impendence to movement characteristics”, and if and when this apparatus is added to a violin it will resonate longer, project more sound frequencies further, produce a stronger response, and require half the bowing effort.

US Patent:

57601895, Jun 2, 1998

Filed:

Jun 2, 1995

Appl. No.:

8/464176

Inventors:

Steven M. Vicik - Natick MA
Neil L. Schauer - Milford MA
James R. Mercer - Derry NH
Edward R. LaVallie - Tewksbury MA
Catherine A. Briasco - Burlington MA
Jeffrey S. Deetz - Melrose MA
Dwight Winters - Camarillo CA
Jenifer L. Thomas - Salem NH

Assignee:

Genetics Institute, Inc. - Cambridge MA

International Classification:

C07K 114
C07K 118
C07K 132
C07K 136

US Classification:

530412

Abstract:

Provided by the present invention are novel methods of protein recovery and purification methods and more specifically novel methods for the recovery and purification thioredoxin fusion proteins, especially of IL-11.

US Patent:

20180077028, Mar 15, 2018

Filed:

Sep 13, 2016

Appl. No.:

15/263653

Inventors:

- New York NY, US
Alex ESTERKIN - Revere MA, US
James B. MERCER - Millville MA, US

Assignee:

CA, Inc. - New York NY

International Classification:

H04L 12/24
G06F 17/30
H04L 12/26

Abstract:

Some embodiments disclosed herein are directed to a capacity management node having a processor and a memory coupled to the processor and comprising computer readable program code that when executed by the processor causes the processor to perform operations including determining, using historical metric data that is used to determine a performance metric projection corresponding to a network computing resource, a performance projection quality factor that indicates a reliability of the performance metric projection based on a plurality of data driven values and a plurality of user specified values that correspond to the plurality of data driven values.