James Lee Morgan

James Morgan Brighton, MA

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Work:

American Personnel, AP Healthcare
Boston, MA
Apr 2014 to Feb 2015
Recruiter JP Morgan & Co Real Estate Appraisers and Consultants
Wakefield, MA
May 2012 to Apr 2014
Assistant Operations Manager

Education:

Boston University
Los Angeles, CA
Sep 2008 to Dec 2008
Graduate Certificate in Film George Washington University
Washington, DC
Sep 2004 to May 2008
Graduate Certificate in Film St John's Preparatory School
Danvers, MA
Sep 2004 to May 2008
High School Diploma

James Morgan Groton, MA

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Work:

Dana Farber Cancer Institute

Feb 2009 to 2000
Staff Scientist/Tech: Director Eisai Research Institute
Lexington, MA
Feb 2005 to Jan 2009
Senior Scientist- Translational Research/Biologics Research Genzyme Corporation
Framingham, MA
Jul 2001 to Jul 2001
Staff Scientist ZYCOS, Inc (acquired by MGI Pharma, Inc.)
Lexington, MA
Sep 1998 to Jul 2001
Senior Research Associate UMASS Medical Center
Worcester, MA
Sep 1996 to Sep 1998
Research Technician Antigen Express, Inc
Worcester, MA
Sep 1995 to Sep 1996
Molecular Immunology Scientist

Education:

Worcester State College
2004
M.S. in Biotechnology Worcester State College
1993
B.S. in Biology

Skills:

Proficient in using cell lines and primary cells, including tissue dissociation, ficoll/percoll cell separations, column and magnetic bead (MACS, Dynal) isolations. Immunologic assays: ELISpot, ELISA, antibody and neutralizing antibody titering, 51Cr release CTL, 3H incorporation (proliferation and suppression, MLRs, etc), dendritic cell, macrophage and long term lymphocyte culture and antigen-specific T cell line cloning, BLCL generation, in vitro immunization (IVI). Other in vitro assays/methods: cell based drug evaluations, histology, IHC, Multiplex (Luminex) assays, enzyme-substrate assays (SPM, -Gal,-Gal, SEAP, etc), XF bioenergenic assays, T-cell hybridoma and vaccinia vector generation, cloning, screening/purification, plaque assays, infections etc., Flow Cytometry (FACS): immunophenotyping (lymphocytes, Tregs, stem cells, alveolar macrophages, tumor and tumor stromal cells, etc.), MHC Class I tetramer staining, intracellular staining (transcription factors, cytokines, phospho-proteins), CFSE dilution, CFSE in vivo CTL assays, phagocytosis and ROS assays. Molecular Bio: immuno-blots, SDS-PAGE, gel electroporesis, plasmid preps, 32/33P in situ hybridization, transfections with electroporation, Fugene, calcium phosphate, etc., TREC RQ-PCR, organic extractions. In vivo studies: pre-clinical POC, PK, ADA and efficacy studies, cell and antibody passive transfer, xenogeneic/syngeneic tumor models, Matrigel angiogenesis, in vivo electroporation, test article injection (IP, IV, SC, IM, IR, IN, PO), anesthesia, disease induction (IBD, UC, EAE, UUO, irradiation), surgeries, blood collection, whole body perfusion, peritoneal and bronchoalveolar lavage, tissue harvests and preparation, IACUC Chair, vivarium management and transgenic mouse breeding colony maintenance.