James M Oakley

US Patent:

20220306683, Sep 29, 2022

Filed:

Jun 5, 2020

Appl. No.:

17/616117

Inventors:

- Princeton NJ, US
- Cambridge MA, US
Tao WANG - Princeton NJ, US
James OAKLEY - Princeton NJ, US
Tamara REYES-ROBLES - Boston MA, US
Rob C. OSLUND - Brookline MA, US
Olugbeminiyi O. FADEYI - Harvard MA, US
Dann LeRoy Parker - Cranford NJ, US
Frances Paola RODRIGUEZ-RIVERA - Jersey City NJ, US
Stefan MCCARVER - San Diego CA, US

International Classification:

C07K 1/13
G01N 33/533
G01N 33/542
G01N 33/58
G01N 33/68

Abstract:

In one aspect, compositions and methods are described herein for providing a microenvironment mapping platform operable to selectively identify various features, including protein-protein interactions on cellular membranes. In some embodiments, a composition comprises a catalyst, and a protein labeling agent, wherein the catalyst activates the protein labeling agent to a reactive intermediate. The catalyst, in some embodiments, can have electronic structure for permitting energy transfer to the protein labeling agent to form the reactive intermediate. The reactive intermediate reacts or crosslinks with a protein or other biomolecule within the diffusion radius of the reactive intermediate.