James A Pierce

US Patent:

55893643, Dec 31, 1996

Filed:

Jul 29, 1994

Appl. No.:

8/282030

Inventors:

Jon I. Williams - Robbinsville NJ
James C. Pierce - Wilmington DE
G. Mark Anderson - Norristown PA
Prasad Kari - Lansdale PA

Assignee:

Magainin Pharmaceuticals Inc. - Plymouth Meeting PA

International Classification:

C12P 2100

US Classification:

435 697

Abstract:

The present invention relates to the recombinant production of amphiphilic peptides with biologically and therapeutically significant activities. In one embodiment, this invention relates to recombinantly producing an amphiphilic peptide by providing a protease-deficient microbial host transformed with an expression vector containing DNA that encodes the amphiphilic peptide under the control of a regulatory sequence operable in the microbial host and expressing the amphiphilic peptide in the transformed microbial host. In another embodiment, this invention relates to providing an E. coli protease-deficient K-12 cell transformed with a vector that expresses a cleavable fusion protein comprising at least part of a carbohydrate binding protein and the amphiphilic peptide in the cell, expressing the fusion protein in the cell, and cleaving the fusion protein to obtain the amphiphilic peptide substantially free of carbohydrate binding protein residues. The biologically active amphiphilic peptide so produced can be further treated chemically or enzymatically to obtain a chemically distinct amphiphilic peptide with improved biological and therapeutic properties.