James S Schaefer

US Patent:

6635463, Oct 21, 2003

Filed:

Jan 11, 2001

Appl. No.:

09/758282

Inventors:

Wu-Po Ma - Madison WI
Victor I. Lyamichev - Madison WI
Michael W. Kaiser - Madison WI
Hatim Taysir Allawi - Madison WI
James J. Schaefer - Madison WI
Bruce P. Neri - Madison WI

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C12N 922

US Classification:

435199, 536 232

Abstract:

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

US Patent:

6759226, Jul 6, 2004

Filed:

May 24, 2000

Appl. No.:

09/577304

Inventors:

Wu-Po Ma - Madison WI
Victor I. Lyamichev - Madison WI
Michael W. Kaiser - Madison WI
Hatim Taysir Allawi - Madison WI
James J. Schaefer - Madison WI
Bruce P. Neri - Madison WI

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C12N 922

US Classification:

435199, 435 911

Abstract:

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

US Patent:

7045289, May 16, 2006

Filed:

May 24, 2001

Appl. No.:

09/864636

Inventors:

Hatim Allawi - Madison WI, US
Christian Tor Bartholomay - Madison WI, US
LuAnne Chehak - Janesville WI, US
Michelle L. Curtis - Cottage Grove WI, US
Peggy S. Eis - San Diego CA, US
Jeff G. Hall - Madison WI, US
Hon S. Ip - Madison WI, US
Michael Kaiser - Madison WI, US
Andrew A. Lukowiak - Stoughton WI, US
Victor Lyamichev - Madison WI, US
WuPo Ma - Madison WI, US
Sarah M. Olson - Cross Plains WI, US
James J. Schaefer - Madison WI, US
Zbigniew Skrzypczynski - Verona WI, US
Tsetska Y. Takova - Madison WI, US
Kevin L. Vedvik - Madison WI, US
Natalie Lyamichev - Madison WI, US
Bruce P. Neri - Carlsbad CA, US

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent:

7150982, Dec 19, 2006

Filed:

Feb 26, 2002

Appl. No.:

10/084839

Inventors:

Hatim T. Allawi - Madison WI, US
Brad T. Argue - Sun Prairie WI, US
Christian Tor Bartholomay - Madison WI, US
LuAnne Chehak - Janesville WI, US
Michelle L. Curtis - Cottage Grove WI, US
Peggy S. Eis - San Diego CA, US
Jeff G. Hall - Madison WI, US
Hon S. Ip - Madison WI, US
Lin Ji - Madison WI, US
Michael W. Kaiser - Madison WI, US
Robert W. Kwiatkowski - Verona WI, US
Andrew A. Lukowiak - Stoughton WI, US
Victor Lyamichev - Madison WI, US
WuPo Ma - Madison WI, US
Bruce P. Neri - Carlsbad CA, US
Sarah M. Olson - Cross Plains WI, US
James J. Schaefer - Madison WI, US
Zbigniev Skrzypczynski - Verona WI, US
Tsetska Y. Takova - Madison WI, US
Lisa C. Thompson - Madison WI, US
Kevin L. Vedvik - Madison WI, US

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C12N 9/22

US Classification:

435199, 435 6

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent:

7807806, Oct 5, 2010

Filed:

Oct 30, 2007

Appl. No.:

11/929404

Inventors:

Hatim T. Allawi - Madison WI, US
Brad T. Argue - Sun Prairie WI, US
Christian Tor Bartholomay - Ames IA, US
LuAnne Chehak - Janesville WI, US
Michelle L. Curtis - Cottage Grove WI, US
Peggy S. Eis - Fitchburg WI, US
Jeff G. Hall - Waunakee WI, US
Hon S. Ip - Madison WI, US
Lin Ji - Madison WI, US
Michael W. Kaiser - Madison WI, US
Robert W. Kwiatkowski - Hopewell Junction NY, US
Andrew A. Lukowiak - Stoughton WI, US
Victor Lyamichev - Madison WI, US
Wupo Ma - Madison WI, US
Bruce P. Neri - Carlsbad CA, US
Sarah M. Olson - Cross Plains WI, US
James J. Schaefer - Madison WI, US
Zbigniev Skrzypczynski - Verona WI, US
Tsetska Y. Takova - Madison WI, US
Lisa C. Thompson - Madison WI, US
Kevin L. Vedvik - Madison WI, US

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C07H 21/04
C07K 14/00

US Classification:

536 231, 530350

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent:

7935800, May 3, 2011

Filed:

Dec 19, 2006

Appl. No.:

11/642815

Inventors:

Hatim T. Allawi - Madison WI, US
Brad T. Argue - Sun Prairie WI, US
Christian Tor Bartholomay - Madison WI, US
LuAnne Chehak - Janesville WI, US
Michelle L. Curtis - Cottage Grove WI, US
Peggy S. Eis - San Diego CA, US
Jeff G. Hall - Madison WI, US
Hon S. Ip - Madison WI, US
Lin Ji - Madison WI, US
Michael W. Kaiser - Madison WI, US
Robert W. Kwiatkowski - Verona WI, US
Andrew A. Lukowiak - Stoughton WI, US
Victor Lyamichev - Madison WI, US
WuPo Ma - Madison WI, US
Bruce P. Neri - Carlsbad CA, US
Sarah M. Olson - Cross Plains WI, US
James J. Schaefer - Madison WI, US
Zbigniev Skrzypczynski - Verona WI, US
Tsetska Y Takova - Madison WI, US
Lisa C. Thompson - Madison WI, US
Kevin L. Vedvik - Madison WI, US

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C07H 21/02
C12Q 1/68
C07K 1/00

US Classification:

536 231, 435 6, 530350

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent:

8063184, Nov 22, 2011

Filed:

Oct 5, 2010

Appl. No.:

12/898490

Inventors:

Hatim Allawi - Madison WI, US
Brad T. Argue - Sun Prairie WI, US
Christian Tor Bartholomay - Madison WI, US
LuAnne Chehak - Janesville WI, US
Michelle L. Curtis - Cottage Grove WI, US
Peggy S. Eis - Madison WI, US
Jeff G. Hall - Madison WI, US
Hon S. Ip - Madison WI, US
Lin Ji - Madison WI, US
Michael Kaiser - Madison WI, US
Andrew A. Lukowiak - Stoughton WI, US
Victor Lyamichev - Madison WI, US
WuPo Ma - Madison WI, US
Bruce P. Neri - Madison WI, US
Sarah M. Olson - Cross Plains WI, US
James J. Schaefer - Madison WI, US
Zbigniev Skrzypczynski - Verona WI, US
Tsetska Y. Takova - Madison WI, US
Lisa C. Thompson - Madison WI, US
Kevin L. Vedvik - Madison WI, US

Assignee:

Third Wave Technologies, Inc. - Madison WI

International Classification:

C07K 14/00
C12N 15/00

US Classification:

530350, 435 691

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent:

20040018489, Jan 29, 2004

Filed:

May 24, 2001

Appl. No.:

09/864426

Inventors:

WuPo Ma - Madison WI, US
Victor Lyamichev - Madison WI, US
Michael Kaiser - Madison WI, US
Hatin Allawi - Madison WI, US
Andrew Lukowiak - Madison WI, US
James Schaefer - Madison WI, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12P021/02
C12N005/06

US Classification:

435/006000, 435/069100, 435/091200, 435/199000, 435/320100, 435/325000, 536/023200

Abstract:

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.