James J Stachecki

US Patent:

20060046243, Mar 2, 2006

Filed:

Apr 14, 2005

Appl. No.:

11/105918

Inventors:

James Stachecki - Parsippany NJ, US
Steen Willadsen - Windemere FL, US

International Classification:

A01N 1/02
A01N 1/00

US Classification:

435001300

Abstract:

A method of vitrifying mammalian cells. According to the method of the present invention, biological cells of mammalian origin are frozen quickly by a vitrification method. Upon exposure to a coolant, the biological cells undergo vitrification. The biological cells which have undergone vitrification may be stored for a period of time and then devitrified at a later date. The devitrified biological cells remain viable. Preferred biological cells according to the present invention are developmental cells including blastocysts, embryos, and oocytes.

US Patent:

59855380, Nov 16, 1999

Filed:

Aug 1, 1997

Appl. No.:

8/904671

Inventors:

James J. Stachecki - Parsippany NJ

Assignee:

Saint Barnabas Medical Center - Livingston NJ

International Classification:

A01N 102

US Classification:

435 11

Abstract:

A cell culture medium and cryopreservation medium in which sodium chloride is replaced with an organic cation, preferably choline chloride in a concentration of at least 100 mM, resulting a residual sodium ion concentration less than about 50 mM. The cryopreservation solution is suitable for cryopreservation of unfertilized oocytes, with thawed oocytes demonstrating the ability to survive, fertilize, and for the resulting embryos to proceed to full term development.