James L Willey

Address:

24463 Luckey Rd, Perrysburg, OH 43551

VIN:

1GNDV23127D103921

Make:

CHEVROLET

Model:

UPLANDER

Year:

2007

US Patent:

7476502, Jan 13, 2009

Filed:

Apr 11, 2005

Appl. No.:

11/103397

Inventors:

James C. Willey - Toledo OH, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

The present invention is directed to methods and compositions for assaying one or more analytes in a sample with improved sensitivity, reproducibility and/or lower detection limits, as well as applications employing such compositions and methods. In particular, the present invention provides methods and compositions for assaying one or more non-nucleic acid analytes with one or more other nucleic acid and/or non-nucleic acid analytes that serve as controls.

US Patent:

7527930, May 5, 2009

Filed:

Jan 23, 2006

Appl. No.:

11/338860

Inventors:

James C. Willey - Toledo OH, US
Brad Austermiller - Defiance OH, US
Erin L. Crawford - Rossford OH, US
Charles Knight - Toledo OH, US
Terry Osborn - Lake Bluff IL, US
Robert Zahorchak - Madison AL, US

Assignee:

Gene Express, Inc. - Toledo OH
Medical University of Ohio - Toledo OH

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.

US Patent:

8304192, Nov 6, 2012

Filed:

Mar 4, 2005

Appl. No.:

11/072700

Inventors:

James C. Willey - Toledo OH, US
Erin L. Crawford - Rossford OH, US
David A. Weaver - Perrysburg OH, US

Assignee:

University of Toledo - Toledo OH

International Classification:

C12Q 1/68

US Classification:

435 612, 435 611

Abstract:

The present invention is directed to methods and compositions for evaluating allelic variations with improved quality controls, methods of preparing such compositions, and applications employing such compositions and methods. In particular, the present invention provides methods and compositions for reducing false positives and/or false negatives in nucleic acid measurements.

US Patent:

8563238, Oct 22, 2013

Filed:

Jun 4, 2007

Appl. No.:

11/757705

Inventors:

James C. Willey - Toledo OH, US
David A. Weaver - Perrysburg OH, US
Erin L. Crawford - Rossford OH, US

Assignee:

University of Toledo - Toledo OH

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 61, 435 912, 536 231, 536 243

Abstract:

A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed.

US Patent:

20030186246, Oct 2, 2003

Filed:

Mar 28, 2002

Appl. No.:

10/109349

Inventors:

James Willey - Toledo OH, US
Erin Crawford - Rossford OH, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

A method for direct comparison of numerical gene expression values between samples of genes using reverse transcription-polymerase chain reaction is described. cDNA, a competitive template mixture, and primer pairs for a plurality of genes are combined with at least one suitable buffer and at least one suitable enzyme to form a mixture. The mixture is amplified for a predetermined number of cycles to form PCR products. The PCR products are mixed with at least one suitable buffer, at least one enzyme, and one primer pair specific for each of the genes. The resulting mixture is amplified an additional predetermined number of cycles.

US Patent:

20040197785, Oct 7, 2004

Filed:

Mar 11, 2004

Appl. No.:

10/471473

Inventors:

James Willey - Toledo OH, US
David Weaver - Perrysburg OH, US
Erin Crawford - Rossford OH, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed.

US Patent:

20050260586, Nov 24, 2005

Filed:

Mar 27, 2003

Appl. No.:

10/508932

Inventors:

Jeffrey Demuth - Blanchester OH, US
Erin Crawford - Rossford OH, US
James Willey - Toledo OH, US
David Weaver - Perrysburg OH, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

The present invention identifies and quantifies changes in gene expression associated with non-small cell lung cancer NSCLC by examining gene expression in tissue from normal lung and diseased lung. The present invention also identifies and quantifies expression profiles which serve as useful diagnostic markers as well as markers that are useful to monitor disease states, disease progression, drug toxicity, drug efficacy and drug metabolism.

US Patent:

20060183144, Aug 17, 2006

Filed:

Jan 23, 2006

Appl. No.:

11/338859

Inventors:

James Willey - Toledo OH, US
Brad Austermiller - Defiance OH, US
Erin Crawford - Rossford OH, US
Charles Knight - Toledo OH, US
Terry Osborn - Lake Bluff IL, US
Robert Zahorchak - Madison IL, US

Assignee:

Medical College of Ohio - Toledo OH
Gene Express, Inc. - Toledo OH

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.