Jamie Danyale Walker

US Patent:

20180348205, Dec 6, 2018

Filed:

May 24, 2018

Appl. No.:

15/988421

Inventors:

- Basel, CH
Fan Zhang - Keller TX, US
Denise P. Rodeheaver - Fort Worth TX, US
Ann Marie Wright - Roswell GA, US
Charion Tolliver - Fort Worth TX, US
Jamie Michaelis Walker - Burleson TX, US
Robert Edward Rose - North Richlands Hills TX, US
Stephen Paul Shannon - Arlington TX, US
Jeffrey Charles White - Southlake TX, US

International Classification:

G01N 33/50

Abstract:

A cytotoxicity method for medical devices that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) direct contact method is developed. The quantitative, direct contact cytotoxicity test method uses Mouse lymphoma TK or human lymphoblastoid TK6 suspension cells for medical device safety assessment. The advantages of using the suspension cell assay over adherent V79-4 cells is that this approach is quantitative, automated, not susceptible to potential mechanical damage imparted by the overlying test materials, and avoids issues related to non-adherence of cells to the test material which might impact the interpretation of test results.