Janice T Jones

US Patent:

20210207152, Jul 8, 2021

Filed:

Mar 19, 2021

Appl. No.:

17/206484

Inventors:

- Durham NC, US
JANICE C. JONES - Apex NC, US
MICHAEL G. KOZIEL - Raleigh NC, US
SCOTT JOSEPH UKNES - Apex NC, US

Assignee:

AGBIOME, INC. - Durham NC

International Classification:

C12N 15/78
C12N 1/36
C12R 1/39
A01N 37/46
A01N 43/54
A01N 43/653
A01N 57/20
A01N 63/00

Abstract:

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. The modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. Modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations can be added to soils to prevent fungal pathogens and associated diseases, promoting plant growth. The present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Compositions include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or as a seed coating for plants and seeds.

US Patent:

20210130811, May 6, 2021

Filed:

Jan 13, 2021

Appl. No.:

17/148210

Inventors:

- DURHAM NC, US
JANICE JONES - APEX NC, US
JESSICA PARKS - RALEIGH NC, US
REBECCA E. THAYER - MORRISVILLE NC, US
DANIEL J. TOMSO - BAHAMA NC, US
SCOTT JOSEPH UKNES - APEX NC, US
SANDY VOLRATH - DURHAM NC, US
ERIC RUSSELL WARD - DURHAM NC, US

Assignee:

AGBIOME, INC. - DURHAM NC

International Classification:

C12N 15/10
C12Q 1/6869
C12Q 1/6806
C12Q 1/6827

Abstract:

Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.

US Patent:

20200048643, Feb 13, 2020

Filed:

Oct 21, 2019

Appl. No.:

16/659186

Inventors:

- Research Triangle Park NC, US
Janice C. Jones - Apex NC, US
Michael G. Koziel - Raleigh NC, US
Scott Joseph Uknes - Apex NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

C12N 15/78
A01N 63/00
A01N 57/20
A01N 43/653
A01N 43/54
A01N 37/46
C12R 1/39
C12N 1/36

Abstract:

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. The modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. Modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations can be added to soils to prevent fungal pathogens and associated diseases, promoting plant growth. The present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Compositions include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or as a seed coating for plants and seeds.

US Patent:

20190316145, Oct 17, 2019

Filed:

May 17, 2019

Appl. No.:

16/415197

Inventors:

- Research Triangle Park NC, US
James R. Henriksen - Cary NC, US
Janice C. Jones - Apex NC, US
Rebekah Deter Kelly - Durham NC, US
Amy Shekita - Cary NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 9/10
A01N 57/20

Abstract:

Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

US Patent:

20190021336, Jan 24, 2019

Filed:

Sep 27, 2018

Appl. No.:

16/143884

Inventors:

- Research Triangle Park NC, US
Janice C. Jones - Apex NC, US
Michael G. Koziel - Raleigh NC, US
Amy Elizabeth Shekita - Cary NC, US
Kelly S. Smith - Durham NC, US
Scott Joseph Uknes - Apex NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

A01N 63/00
A01N 43/653
C12N 1/36
A01N 37/22
A01N 25/08
A01N 37/34
C12N 15/78
C12N 9/10
A01N 25/14
A01N 43/40
A01N 57/20
C12R 1/39
A01N 25/00
A01N 43/54

Abstract:

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. The improved, modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. Modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations can be added to soils to prevent fungal pathogens and the associated diseases thereby promoting plant growth. The present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Disclosed compositions include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or seed coating for plants and seeds.

US Patent:

20180289014, Oct 11, 2018

Filed:

Jun 8, 2018

Appl. No.:

16/004023

Inventors:

- Research Traingle Park NC, US
Janice C. Jones - Apex NC, US
Michael G. Koziel - Raleigh NC, US
Scott Joseph Uknes - Apex NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

A01N 63/00
A01N 43/653
C12N 1/36
A01N 37/22
A01N 25/08
A01N 37/34
C12N 15/78
C12N 9/10
A01N 25/14
A01N 43/40
A01N 57/20
C12R 1/39
A01N 25/00
A01N 43/54

Abstract:

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. The improved, modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. Modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations can be added to soils to prevent fungal pathogens and the associated diseases thereby promoting plant growth. The present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Disclosed compositions include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or seed coating for plants and seeds.

US Patent:

20180135045, May 17, 2018

Filed:

Jan 4, 2018

Appl. No.:

15/862184

Inventors:

- Research Triangle Park NC, US
Janice Jones - Apex NC, US
Jessica Parks - Raleigh NC, US
Rebecca E. Thayer - Morrisville NC, US
Daniel J. Tomso - Bahama NC, US
Scott Joseph Uknes - Apex NC, US
Sandy Volrath - Durham NC, US
Eric Russell Ward - Durham NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

C12N 15/10
C12Q 1/6827
C12Q 1/6869
C12Q 1/6806

Abstract:

Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.

US Patent:

20180116227, May 3, 2018

Filed:

Oct 19, 2017

Appl. No.:

15/788370

Inventors:

- Research Triangle Park NC, US
Janice C. Jones - Apex NC, US
Michael G. Koziel - Raleigh NC, US
Amy Elizabeth Shekita - Cary NC, US
Kelly S. Smith - Durham NC, US
Scott Joseph Uknes - Apex NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

A01N 63/00
C12R 1/39
A01N 37/34
A01N 25/08
A01N 57/20
A01N 43/40
C12N 15/78
C12N 9/10
A01N 43/54
A01N 25/14
A01N 37/22
A01N 25/00
A01N 43/653
C12N 1/36

Abstract:

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. By improving the population of biological agents, the modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. In particular, modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations of biological agents can be added to soils to prevent fungal pathogens and the diseases they cause promoting plant growth. Therefore, the present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Compositions of the invention include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or as a seed coating for plants and seeds.