Thomas J. Wickham - Bethesda MD Erik Falck-Pedersen - Dobbs Ferry NY Petrus W. Roelvink - Gaithersburg MD Joseph T. Bruder - New Market MD Jason Gall - New York NY Imre Kovesdi - Rockville MD
Assignee:
Cornell Research Foundation, Inc. - Ithaca NY GenVec, Inc. - Gaithersburg MD
The invention provides a chimeric adenovirus fiber protein including a nonnative amino acid sequence, and a chimeric adenovirus fiber protein lacking a native amino acid receptor-binding sequence. The chimeric protein trimerizes when produced in a mammalian cell.
Non-Adenoviral Gene Product-Based Complementing Cells For Adenoviral Vectors
Douglas Brough - Gaithersburg MD, US Jason Gall - Germantown MD, US Imre Kovesdi - Rockville MD, US
Assignee:
GenVec, Inc. - Gaithersburg MD
International Classification:
C12N015/861 C12N007/01 C12N005/08
US Classification:
435/456000, 435/235100, 435/320100, 435/366000
Abstract:
The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.
Douglas Brough - Gaithersburg MD, US Jason Gall - Germantown MD, US Imre Kovesdi - Rockville MD, US
Assignee:
GenVec, Inc. - Gaithersburg MD
International Classification:
C12N015/861 C12N007/00 C12N005/08
US Classification:
435/456000, 435/235100, 435/366000, 435/320100
Abstract:
The invention provides cells, particularly NCI-H460 cells and Calu-1 cells, for the propagation of replication-deficient adenoviral vectors. The cells are lung carcinomas with either a wild-type p53 gene or a heterozygous K-ras mutation. The cells comprise at least one adenoviral nucleic acid sequence, which upon expression produces a gene product that complements for at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.
Chimeric Adenoviral Fiber Protein And Methods Of Using Same
Thomas Wickham - Germantown MD, US Erik Falck-Pedersen - Dobbs Ferry NY, US Petrus Roelvink - Germantown MD, US Joseph Bruder - Ijamsville MD, US Jason Gall - Germantown MD, US Imre Kovesdi - Rockville MD, US
Assignee:
Cornell Research Foundation, Inc. - Ithaca NY
International Classification:
C12N015/861 C12N007/00
US Classification:
435/456000, 435/235100
Abstract:
A recombinant adenovirus comprising a chimeric adenoviral fiber protein comprising a non-adenoviral amino acid sequence in place of or in addition to a native fiber amino acid sequence, wherein the chimeric adenoviral fiber protein comprises a trimerization domain and the non-adenoviral amino acid sequence is of a size such that folding of the chimeric adenoviral fiber protein and assembly of a complex comprising the chimeric adenoviral fiber protein and a penton base is not impeded, and an adenoviral transfer vector for the generation of such a recombinant adenovirus are provided.
Non-Adenoviral Gene Product-Based Complementing Cells For Adenoviral Vectors
Douglas Brough - Gaithersburg MD, US Jason Gall - Germantown MD, US Imre Kovesdi - Rockville MD, US
Assignee:
GenVec, Inc. - Gaithersburg MD
International Classification:
C12N005/08 C12N015/861
US Classification:
435/369000, 435/456000
Abstract:
The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.
Jason Gall - Germantown MD, US Thomas Wickham - Billerica MA, US William Enright - North Potomac MD, US Douglas Brough - Gaithersburg MD, US Mohammed Zuber - Fredrick MD, US C. King - Washington DC, US Gary Nabel - Bethesda MD, US Cheng Cheng - Bethesda MD, US
International Classification:
A61K 39/12 A61K 39/395
US Classification:
424199100, 424204100, 424130100
Abstract:
The invention provides a method of inducing an immune response in a mammal. The method comprises administering to the mammal a non-subgroup C adenoviral vector comprising an adenoviral fiber protein having an amino acid sequence comprising about 80% or more identity to an amino acid sequence encoding a subgroup C adenoviral fiber protein. The adenoviral vector further comprises a nucleic acid sequence encoding an antigen which is expressed in the mammal to induce an immune response. The invention further comprises a method of producing an adenoviral vector, and a composition comprising a serotype 41 or a serotype 35 adenoviral vector and a carrier. The invention also provides an adenoviral vector comprising a nucleic acid sequence encoding an adenoviral pIX protein operably linked to a heterologous expression control sequence, as well as a method of enhancing the stability and/or packaging capacity of an adenoviral vector.
Method Of Using Adenoviral Vectors To Induce An Immune Response
Gary Nabel - Washington DC, US Cheng Cheng - Bethesda MD, US Wing-Pui Kong - Gaitersburg MD, US Jason Gall - Germantown MD, US C. Richter King - Washington DC, US
Assignee:
GenVec, Inc. - Gaithersburg MD
International Classification:
A61K 39/12 C12N 15/861
US Classification:
424199100, 424093200, 435456000
Abstract:
The invention provides a method of inducing an immune response against a human immunodeficiency virus (HIV) in a mammal. The method comprises administering to the mammal an adenoviral vector composition comprising one or more adenoviral vectors encoding two or more different HIV antigens, the production of which induces an immune response against HIV in the mammal. The invention also provides an adenoviral vector composition comprising four adenoviral vectors encoding an HIV clade A Env protein, an HIV clade B Env protein, an HIV clade C Env protein, and a fusion protein comprising an HIV clade B Gag protein and Pol protein, respectively.
Adenovirus Fiber Shaft Composition And Methods Of Use
The invention provides a gene transfer vector and a conjugate comprising at least three contiguous amino acids of a shaft region of a subgroup C adenovirus fiber protein. The invention also provides methods of using the gene transfer vector and the conjugate to induce an immune response in a mammal, and to deliver a protein or a non-proteinaceous molecule to a specific cell type.
Name / Title
Company / Classification
Phones & Addresses
Jason Gall Production Director
Tta, Inc.
3300 N Central Ave #1520, Phoenix, AZ 85012 4809901101