Jason Wyatt Holder

US Patent:

20120171735, Jul 5, 2012

Filed:

Jun 15, 2010

Appl. No.:

13/378479

Inventors:

Anthony John Sinskey - Boston MA, US
Daniel MacEachran - Cambridge MA, US
Kazuhiko Kurosawa - Somerville MA, US
Paolo Boccazzi - Cambridge MA, US
Jason W. Holder - Brookline MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12P 7/64
C12N 9/02
C07K 14/195
C12N 1/21

US Classification:

435134, 4352523, 435189, 530350

Abstract:

The invention relates to production of triacylglycerols in cells.

US Patent:

20130122549, May 16, 2013

Filed:

Sep 26, 2012

Appl. No.:

13/627060

Inventors:

Michael Sandor Koeris - Natick MA, US
Brett Smith Chevalier - Malden MA, US
Jason Wyatt Holder - Somerville MA, US
Gregory John McKenzie - Arlington MA, US
Daniel Robert Brownell - Arlington MA, US

Assignee:

SAMPLE6 TECHNOLOGIES, INC. - Boston MA

International Classification:

C12P 19/34

US Classification:

435 911, 4352351, 4352551

Abstract:

This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

US Patent:

20200157491, May 21, 2020

Filed:

Nov 15, 2019

Appl. No.:

16/685248

Inventors:

- Cambridge MA, US
Nicolas Mesyngier - Ann Arbor MI, US
Ken Kotz - Newton MA, US
Georgiana Kourepenos - Cambridge MA, US
Jason O. Fiering - Cambridge MA, US
Jason W. Holder - Swampscott MA, US

International Classification:

C12N 1/20
B01L 3/00
C12Q 1/04
C12N 1/06
B01D 21/28
B01D 21/00

Abstract:

A method for preparing and processing a sample is provided. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow over the cells.

US Patent:

20190330599, Oct 31, 2019

Filed:

Jul 12, 2019

Appl. No.:

16/510643

Inventors:

- Cambridge MA, US
Jason Holder - Swampscott MA, US

International Classification:

C12N 7/00
C12N 15/70
C12Q 1/70
C12Q 1/18

Abstract:

The present disclosure provides compositions including recombinant K1E bacteriophages, methods for making the same, and uses thereof. The recombinant K1E bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

US Patent:

20190309265, Oct 10, 2019

Filed:

Jun 20, 2019

Appl. No.:

16/447209

Inventors:

- Cambridge MA, US
Georgiana Kourepenos - Acton MA, US
Parker Dow - Boston MA, US
Jason Holder - Swampscott MA, US

International Classification:

C12N 7/00
C12Q 1/04
C12Q 1/02
C12N 15/74

Abstract:

The present disclosure provides compositions including recombinant B 11 bacteriophages, methods for making the same, and uses thereof. The recombinant B 11 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

US Patent:

20190078133, Mar 14, 2019

Filed:

Sep 6, 2018

Appl. No.:

16/123875

Inventors:

- Cambridge MA, US
Quin Christensen - Cambridge MA, US
Kenneth T. Kotz - Cambridge MA, US
Jason Holder - Swampscott MA, US

International Classification:

C12Q 1/10
C12Q 1/18
C12N 7/00

Abstract:

The present disclosure provides compositions and methods for identifying bacteria and profiling their antibiotic susceptibility. In particular, the methods and compositions of the present technology permit the detection of low concentrations of bacterial cells (e.g.,

US Patent:

20190048325, Feb 14, 2019

Filed:

Aug 7, 2018

Appl. No.:

16/057416

Inventors:

- Cambridge MA, US
Miles T. Rogers - Boston MA, US
Connor McBrine - Somerville MA, US
Jason W. Holder - Swampscott MA, US

International Classification:

C12N 7/02
C12N 15/63

Abstract:

The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

US Patent:

20180346905, Dec 6, 2018

Filed:

May 31, 2018

Appl. No.:

15/994002

Inventors:

- Cambridge MA, US
Jason Holder - Swampscott MA, US

International Classification:

C12N 15/10
C12N 9/22
C12N 15/113

Abstract:

The present disclosure provides methods of generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a linear bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.